苗药健脑安神胶囊对睡眠剥夺大鼠行为及其白细胞介素-1和褪黑素及5-羟色胺含量的影响  

Effect of Jiannao Anshen Capsule of Miao Medicine on the Behavioral Performance,IL-1,MT and 5-HT of Rats with Sleep Deprivation

作　　者：潘晓蓉[1] 余莹[2] 杨琳[2] 

机构地区：[1]贵阳中医学院第二附属医院医务部,贵阳550001 [2]贵阳中医学院研究生院,贵阳550002

出　　处：《医药导报》2017年第4期375-378,共4页Herald of Medicine

基　　金：贵州省科技厅项目(黔科合J字【2013】2075号)

摘　　要：目的探讨苗药健脑安神胶囊对睡眠剥夺大鼠行为及细胞因子白细胞介素-1(IL-1)、褪黑素(MT)及5-羟色胺(5-HT)含量的影响。方法 60只雄性SD大鼠随机分为正常对照组、模型对照组、艾司唑仑组、枣仁安神组、苗药健脑安神组,每组12只。采用对氯苯丙氨酸(PCPA)腹腔注射进行睡眠剥夺,造模成功后分别予相应药物灌胃,观察大鼠的行为学改变,测定大鼠血清5-HT、IL-1、MT及脑组织中5-HT、MT的含量。结果 PCPA所致失眠大鼠行为学积分明显降低,血清中5-HT、MT、IL-1含量及脑组织中5-HT、MT含量明显降低,与正常对照组比较,差异有统计学意义(P<0.05)。苗药健脑安神组、艾司唑仑组、枣仁安神组治疗后失眠大鼠行为学积分明显升高,血清中5-HT、MT、IL-1含量及脑组织5-HT、MT含量明显升高,与模型对照组比较,差异有统计学意义(P<0.05)。苗药健脑安神组、艾司唑仑组、枣仁安神组治疗后失眠大鼠行为学积分、血清中5-HT、MT、IL-1含量及脑组织中5-HT、MT的含量组间比较,差异无统计学意义(P>0.05)。结论苗药健脑安神胶囊可改善睡眠剥夺大鼠的睡眠质量,其机制可能与提高IL-1、MT及5-HT的含量有关。Objective To probe into the influence of Jiannao Anshen Capsule of Miao medicine on the behavioral performance, content of cytokine interleukin-1 （ IL-1 ） , neuroendocrine hormone melatonin （ MT ） and monoamine neurotransmitter 5-hydroxytryptamine （5-HT） of rats with sleep deprival. Methods All of the 60 rats were evenly and randomly divided into 5 groups： the normal control group, model control group, estazolam group, Zaoren Anshen group, and Jiannao Anshen of Miao medicine group, respectively. Every group had 12 rats. Sleep deprivation rat model was established by intraperitoneal injection of para-chlorophenyla lanice （ PCPA）. After successful modeling, the rats were intragastrically administrated with corresponding medicines. The contents of serum 5-HT, IL-1 and MT and the contents of 5-HT, MT in brain tissue were determined. Results Behavioral score, the contents of 5-HT, MT and IL-1 in serum and the contents of 5-HT, MT in brain tissue in insomnia rats caused by PCPA were significantly decreased as compared with normal control group （ P 〈 0. 05 ）. In the Jiannao Anshen group, estazolam group and Zaoren Anshen group, the behavioral score, the content of 5-HT, MT and IL-1 in serum and the content of 5-HT and MT in brain tissue of insomnia rats were significantly increased as compared with model control group （ P 〈 0. 05 ） . The behavioral score, the content of 5-HT, MT and IL-1 in serum and the content of 5-HT and MT in brain tissue of insomnia rats were not statistically significant different among Jiannao Anshen group, estazolam group and Zaoren Anshen group （ P 〉 0. 05 ） . Conclusion Jiannao Anshen capsule of Miao medicine could obviously improve the sleep quality of rats with sleep deprival. The mechanism may be related to improving the content of IL-1, MT and 5-HT.

关 键 词：健脑安神胶囊 苗药 5-羟色胺 白细胞介素-1 褪黑素 睡眠剥夺 

分 类 号：R285[医药卫生—中药学] R749[医药卫生—中医学]