旱生香茶菜素G对S180细胞抗肿瘤活性  

Antitumor Activity of Xerophilusin G on S180 Cells

作　　者：杨焕芝[1,2] 周轶平[2] 翁稚颖[2] 罗敏[2] 孙汉董[3] 李玛琳[2,4] 

机构地区：[1]昆明市第一人民医院药学部,昆明650011 [2]昆明医科大学药学院暨云南省天然药物药理重点实验室,昆明650500 [3]中国科学院昆明植物研究所,昆明650204 [4]云南中医学院中药学院,昆明650500

出　　处：《医药导报》2017年第4期379-384,共6页Herald of Medicine

基　　金：云南省自然科学基金重点项目子课题(2004C0008Z)

摘　　要：目的研究旱生香茶菜素G对S180细胞体内外抗肿瘤活性及其作用机制。方法采用改良噻唑蓝(MTT)法测定旱生香茶菜素G对S180细胞株增殖的影响;以荷S180肉瘤小鼠为模型,检测旱生香茶菜素G对荷瘤小鼠肉瘤质量、体质量及其主要脏器的影响;通过流式细胞术检测旱生香茶菜素G对S180细胞株和S180肉瘤组织细胞周期的影响;采用脾脏淋巴细胞转化实验法测定旱生香茶菜素G对荷瘤小鼠脾脏淋巴细胞增殖活性;采用酶联免疫吸附试验双抗夹心法检测旱生香茶菜素G对荷瘤小鼠血清中白细胞介素(IL)-2含量的影响。结果旱生香茶菜素G对S180细胞株半数抑制浓度(IC50值)为19.80μg·m L^(-1)。小鼠经腹腔注射旱生香茶菜素G的LD50为121.11 mg·kg^(-1)。在剂量为3,6 mg·kg^(-1)时,对荷S180肉瘤小鼠抑瘤率分别为32.11%,41.60%,差异有统计学意义(P<0.05),小鼠胸腺、肾脏和心脏指数均有一定程度的降低。旱生香茶菜素G使S180细胞株和S180肉瘤组织细胞G0/G1期所占比例增多,对荷瘤小鼠脾脏T、B淋巴细胞具有一定的增殖促进作用(P<0.05)。小、中剂量组血清中IL-2含量分别较对照组减少约90.9%和77.1%(P<0.05)。结论旱生香茶菜素G具有一定的抗肿瘤活性,抑瘤作用可能是通过对肿瘤细胞G0/G1期阻滞,及其促进荷瘤小鼠脾脏T、B淋巴细胞增殖,增强机体免疫功能有关,而与IL-2的表达关系不密切。Objective To study the antitumor activity of Xerophilusin G on SI80 cells,and Its mechanism. Methods Modified MTT assay was used to test the effect of Xerophilusin G on the proliferation of SI 80 tumor cell strain. The influences on tumor growth and immune organs of mice with transplanted sarcoma （SI80） were observed. The cell cycle of SI80 cell lines and mouse sarcoma （SI80） was analyzed by flow cytometry. The lymphocyte proliferation activity of spleen stimulating was tested. The level of IL-2 in serum of mice with transplanted sarcoma （SI80） was measured by ELISA. Results The IC50 of Xerophilusin G in SI 80 cell lines was 19. 80 μg · mL^-1 , the LD50 in mouse for Xerophilusin G was 121. 11 mg ·kg^-1 through intraperitoneal injection. The tumor inhibition rate of Xerophilusin G was 32. 11 % and 41. 60% , respectively at the doses of 3 and 6 mg·kg^-1 （ P 〈 0. 05 ） . Compared with the control, the thymus, kidney and cardiac index were decreased. The cell proportion at G0/G1 phase of mouse sarcoma （S180） was increased. T and B cell proliferation activities in tumor-bearing mice were enhanced （ P 〈0. 05）. As compared with control group, the serum level of IL-2 was decreased 90. 9% and 77. 1% in low- and medium-dose groups, respectively （P 〈 0 .0 5 ） . Conclusion Xerophilusin G has remarkable effects in sarcoma （S180） bearing mice. The antitumor mechanism of Xerophilusin G might be related with G0/G 1 phase arrest of mouse sarcoma （S180） cells and enhancing the activity of T and B cell but not related with increasing the secreting of IL-2.

关 键 词：旱生香茶菜 旱生香茶菜素G S180 细胞周期 免疫增强 

分 类 号：R285.5[医药卫生—中药学] R73[医药卫生—中医学]