HIV-1抗体阳性患者流行病学调查及样本蛋白印迹试验带型分析  

Epidemiological investigation of HIV-1 positive infections and pattern analysis of Western blot results

作　　者：陈鑫莹[1] 唐作红 沈来红[1] 吴万里[1] 封正娟 

机构地区：[1]攀枝花市疾病预防控制中心,四川攀枝花617000 [2]攀枝花市中心血站,四川攀枝花617067

出　　处：《中国病毒病杂志》2017年第1期48-53,共6页Chinese Journal of Viral Diseases

摘　　要：目的分析攀枝花市人群人类免疫缺陷病毒(HIV)-1抗体阳性样本流行病学资料及蛋白印迹试验(Western blot,WB)带型,以期为开展艾滋病防治措施提供科学依据。方法对2012-2015年攀枝花市所属各县(市)医院和疾病预防控制中心、血站送检的2 303份HIV抗体初筛阳性样本进行统计分析,并进行WB带型分析。结果 2 303份HIV抗体初筛阳性样本中,HIV-1阳性2 060份,占89.45%,阴性133份,占5.77%,不确定110份,占4.78%;2 060份HIV-1阳性样本中,以男性为主,占73.01%;以年龄≥50岁较为多见,占28.69%;以初中及以下文化程度为主,占77.33%;以已婚有配偶较为多见,占58.88%;感染途径以异性传播和同性传播为主,分别占69.17%、22.96%;2 060份HIV-1抗体阳性样本的WB条带中,共1 998份样本出现6条带及以上,占96.99%,以抗env基因编码蛋白的抗体阳性率最高,占99.40%,其次为抗pol基因编码蛋白的抗体阳性,占93.79%,抗gag基因编码蛋白的抗体阳性率最低,占75.95%;110份不确定样本中,抗env基因编码蛋白、抗pol基因编码蛋白、抗gag基因编码蛋白的抗体阳性率分别为22.12%、5.76%、21.82%。结论艾滋病筛查实验室应进一步加强规范化管理,消除初筛实验假阳性结果的因素。此外,HIV感染者以男性、已婚有配偶、低学历、高龄较为多见,应加强对此类人群的行为干预。Objective To study the epidemiological characteristics of HIV antibody positive patients and ana lyze the band patterns of Western blot （WB） results. Methods A total of 2 303 cases of initial screening HIV antibody positive samples from the city/county hospitals of Panzhihua city, Center for Disease Control and Prevention and Blood Station at Panzhihua city were collected from 2012 to 2015. The characteristics of HIV-1 positive infections and patterns of Western blot were analyzed. Results Among the 2 303 cases of HIV positive samples screened, 2 060 showed HIV-1 positive （89.45%）, 133 negative （5.77%）, and 110 undetermined （4.78%）. Among the 2 060 HIV1 positive patients, male accounted for 73.01%, ages above 50 years accounting for 28.69% , lower educated cases （junior high school or below） accounting for 77.33%, 58.88% were married. The main routes of infection are heterosexual and homosexual, accounting for 69.17% and 22.96% , respectively. Among the 2 060 cases of H1V-1 positive samples, 1 998 samples showed 6 or more patterns on Western blot （96.99 %）. The highest antibody positive rate was anti-env gene encoded protein （99.40%）, followed by the anti-pol gene encoded protein （93.79%） ; the lowest was the anti-gag gene encoded protein （75.95 %）. Among the 110 undetermined samples, the antibody positive rate of anti-env gene encoded protein, anti-pol gene encoded protein and anti gag gene encoded protein were 22.12% , 5.76% and 21.82%, respectively. Conclusions In Panzhihua city of China, the HIV antibody positive patients are mainly male, married, under educated and older people. Six or more bands are the most common pattern in Western blot analysis.

关 键 词：HIV-1抗体 流行病学分析 蛋白印迹试验 条带 

分 类 号：R512.91[医药卫生—内科学] R181.3[医药卫生—临床医学]