高效液相色谱法测定小儿盐酸二氧异丙嗪冲剂的含量  

Quantitative Determination of Dioxopromethazine Hydrochloride Granules for Children by High Performance Liquid Chromatography

作　　者：赵磊石 李静莉[2] 李太平 

机构地区：[1]牡丹江友搏药业,黑龙江省牡丹江市157000 [2]黑龙江省药品检验所,哈尔滨市150001 [3]丹东市药品检验所,辽宁省丹东市118002

出　　处：《光谱实验室》2004年第3期469-472,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：采用高效液相色谱法测定盐酸二氧异丙嗪的含量。色谱柱为 DIKMATMRPC18(4 .6 mm× 2 0 cm,5 μm) ,流动相为乙腈 - 0 .5 %磷酸二氢钾 -三乙胺 (5 0∶ 5 0∶ 0 .2 ) ,用磷酸调 p H值至 6 .8± 0 .1;流速为1.0 m L/ min,检测波长为 2 6 4nm。该法回收率为 99.6 % ,RSD=0 .6 1%。盐酸二氧异丙嗪在浓度为0 .0 184 8— 0 .0 4 312 mg/ m L范围内线性关系良好。相关系数为 r=0 .9999。该方法操作简便 ,结果准确可靠。A method for the determination of dioxopromethazine hydrochloride by high performance liquid chromatography(HPLC,RP) was established.The operating conditions were Diamonsil TM C18 column (5μ, 4.6mm i.d.×20cm), acetonitrile-0.5% potassium dihydrogen phosphate-triethylamine (55∶50∶0.2 with phosphoric acid to adjust pH 6.8±0.1)as mobile phase,at a flow rate of 1.0 mL/min and UV detection at 264nm.The calibration curve is a linear over the range of 0.01848- 0.04321mg/mL with the corelation coefficient of 0.9999.The average recovery is 99.6% and the RSD is 0.61%.The method is simple,accurate and suitable for routine analysis.

关 键 词：高效液相色谱法 盐酸二氧异丙嗪 

分 类 号：O657.72[理学—分析化学]