Chemotransformation of Bacterial Cells Without Heat-shock  

作　　者：WANG Sainan LI Zhenhua CHEN Zhijun 

机构地区：[1]State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, International Joint Research Laboratory of Nano-micro Architecture Chemistry, Jilin University, Changchun 130012, R R. China

出　　处：《Chemical Research in Chinese Universities》2017年第2期160-165,共6页高等学校化学研究（英文版）

摘　　要：Transformation of DNA into microbial cells via heat-shock approach has been well established in the fieldof molecular biology for decades. Herein we described an unexpected finding that heat-shock may not play an essen-tial role in the transformation process. This observation was verified via UV-Vis and fluorescence spectroscopies, andconfocal microscopy images for various DNAs and bacterial cells. The non-heat-shock approach proposed in thisstudy can be a convenient and beneficial modification for DNA transformation, especially for those laboratorieslacking ice machine and heat-shock equipment.

关 键 词：HEAT-SHOCK Chemotransformation DNA BACTERIA 

分 类 号：O[理学]