高活性小牛血去蛋白提取物对糖尿病大鼠肾损伤的保护作用  

Protective effect of high activity deproteinized extract of calf blood on kidney injury of diabetic rats

作　　者：糜心雅 石立强[1] 李洪宇[1] 苑广信[1] 谢立亚[1] 杜培革[1] 安丽萍[1] MI Xinya SHI Liqiang LI Hongyu YUAN Guangxin XIE Liya DU Peige AN Liping(Department of Microbiology and Biochemistry, School of Pharmacy, Beihua University, Jilin 132013, China)

机构地区：[1]北华大学药学院微生物与生化药学教研室,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2017年第2期293-297,I0004,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技创新与科技成果转化计划项目资助课题(0150312014ZX);吉林省科技厅医药产业发展基金资助课题(20140311084YY);吉林省发改委科研基金资助课题(2014Y103);吉林省卫计委科研基金资助课题(20142078)

摘　　要：目的:观察小牛血去蛋白提取物(DECB)对糖尿病大鼠肾损伤的保护作用,并初步探讨其作用机制。方法:Wistar雄性大鼠腹腔注射STZ(65mg·kg^(-1))建立糖尿病模型,将成模大鼠随机分为模型(M)组、二甲双胍(MMet,105 mg·kg^(-1))组、低剂量DECB联合给药(ML,105 mg·kg^(-1)二甲双胍+94.5mg·kg^(-1) DECB)组、中剂量DECB联合给药(MM,105mg·kg^(-1)二甲双胍+189mg·kg^(-1) DECB)组和高剂量联合给药(MH,105mg·kg^(-1)二甲双胍+378mg·kg^(-1) DECB)组(n=10),另取10只Wistar大鼠作为正常对照(NC)组(n=10)。二甲双胍为经口灌胃给药,DECB为腹腔注射给药,每天1次,共给药8周,NC组和M组大鼠给予等体积的生理盐水。测定各组大鼠体质量、血糖水平,检测各组大鼠血清中高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、尿素氮(BUN)、尿微量白蛋白(UAlb)、尿肌酐(UCr)、血肌酐(SCr)、总胆固醇(TC)、甘油三酯(TG)、尿酸(UA)、谷胱甘肽(GSH)、丙二醛(MDA)水平和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性;HE染色观察各组大鼠肾脏组织病理形态学表现。结果:与NC组比较,M组大鼠体质量降低,空腹血糖、UAlb、UCr、SCr、UA、BUN、LDL-C、TC、TG和MDA水平升高(P<0.05),HDL-C和GSH水平降低(P<0.05),SOD和GSH-Px活性降低(P<0.05)。与M组比较,MM和MH组大鼠体质量升高(P<0.05),血糖、UAlb、UCr、SCr、UA、BUN、LDL-C、TC、TG和MDA水平降低(P<0.05),HDL-C和GSH水平升高(P<0.05),SOD和GSH-Px活性升高(P<0.05)。肾脏组织病理观察,与NC组比较,M组大鼠肾脏组织病变明显,肾小球充血,肾小管水肿;与M组比较,各给药组大鼠肾脏组织病变明显好转,肾小管空泡变性减轻,间质增生不明显。结论:DECB联合二甲双胍给药可降低糖尿病大鼠血糖,调节血脂,改善糖尿病大鼠肾组织病理学变化,减轻肾脏损伤,提高机体的抗氧化能力。Objective：To observe the protective effect of deproteinized extract of calf blood（DECB）on the kidney injury of the diabetic rats,and to discuss its mechanism preliminarily.Methods：The male Wistar rats were intraperitoneally injected with STZ（65 mg·kg^-1）to establish the diabetes models,then the model rats were randomly divided into model（M）group,and metformin（MMet,105 mg·kg^-1）group,low dose of combined administration（ML,105 mg·k-1 metformin＋94.5 mg·kg^-1,DECB）group,medium dose of combined administration（MM,105 mg· kg^-1 metformin＋ 189 mg· kg^-1 DECB）group,high dose of combined administration（MH,105mg·kg^-1 metformin ＋378 mg·kg^-1,DECB）group,another ten Wistar rats were selected as normal control（NC）group. The rats were intragastrically administed with metformin and intraperitoneally injected with DECB,once a day,total of 8weeks.The rats in NC group and M group were given normal saline solution.The weights and blood glucose levels of the rats in various groups were determined;the levels of serum high density lipoprotein-cholesterol（HDL-C）,low density lipoprotein-cholesterol（LDL-C）,blood urea nitrogen（BUN）,urinary albumin（UAlb）,urine creatinine（UCR）,serum creatinine（SCr）,total cholesterol（TC）,triglyceride（TG）,uric acid（UA）,glutathione（GSH）,and malondialdehyde（MDA）were detected;the activities of superoxide dismutase（SOD）and glutathione peroxidase（GSH-Px）were determined.The pathological changes of kidney tissue of the rats in various groups were detected by HE staining.Results：Compared with NC group,the weight of the rats in M group was reduced（P〈0.05）,and the levels of blood glucose,UAlb,UCr,SCr,UA,BUN,LDL-C,TC,TG,and MDA were increased（P〈0.05）;the levels of HDL-C and GSH were obviously reduced（P〈0.05）,and the activities of SOD adn GSH-Px were obviously reduced（P〈0.05）.Compared with M group,the weights of the rats in MM and MH groups were increased（P〈0.05）,and the levels of blood glu

关 键 词：小牛血去蛋白提取物 糖尿病肾病 抗氧化 

分 类 号：R587.1[医药卫生—内分泌]