蒙药成分复方对乳房炎模型小鼠治疗作用的研究  

Therapeutic Effect of Mongolian Medicine Composition Compound on Mastitis in Mice

作　　者：高瑞娟[1] 王纯洁[1] 敖日格乐[2] 贾知锋 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018

出　　处：《畜牧与饲料科学》2017年第3期13-16,共4页Animal Husbandry and Feed Science

基　　金：国家自然科学基金项目(31260590);国家科技支撑项目(2015BAD29B06)

摘　　要：旨在探讨蒙药成分复方对乳房炎模型小鼠的治疗作用。利用乳腺内注射大肠杆菌和金黄色葡萄球菌混合菌悬液的方法制备小鼠乳房炎病理模型;模型制备24 h后,阳性对照组、阴性对照组、蒙药成分复方组分别灌胃环丙沙星、生理盐水、蒙药成分复方水溶液,连续给药3 d,每天1次;于注射菌液后24 h(治疗前)和给药后72 h(治疗后),观察各组小鼠的临床症状、组织病理学变化,测定母鼠乳腺组织匀浆中的TNF-α、IL-6含量和乳腺内菌落数。结果表明,蒙药成分复方可以修复由大肠杆菌和金黄色葡萄球菌感染所造成的乳腺组织损伤,显著降低乳房炎模型小鼠乳腺组织中的TNF-α、IL-6含量和乳腺菌落数(P<0.05)。蒙药成分复方可以治疗由大肠杆菌和金黄色葡萄球菌混合感染引起的小鼠乳房炎。The aim of the present study was to evaluate the therapeutic effect of Mongolian medicine composition compound on mastitis in mice. The mastitis model was prepared by intrathymic injection of mixed culture suspension of Escherichia coli（E.coli） and Staphylococcus aureus（S. aureus）. At 24 h after challenge,the mice in positive control group,negative control group,and Mongolian medicine composition compound group were given ciprofloxacin,normal saline,and Mongolian medicine composition compound by intragastric administration once daily for 3 consecutive days. At the 24 h after challenge（pre-treatment） and 72 h after administration（post-treatment）,the clinical symptom and histopathological change of mammary gland of the mice were observed,and the content of TNF-α and IL-6 in mammary gland tissue homogenates and the total bacterial count in mammary gland were determined. The results showed that the Mongolian medicine composition compound was able to repair the injury of the mammary gland caused by co-infection of E. coli and S. aureus and significantly reduce the content of TNF-α and IL-6 as well as total bacterial count in mammary gland of mastitis modle mice（P〈0.05）. The Mongolian medicine composition compound shows therapeutic effect on mastitis in mice caused by co-infection of E. coli and S. aureus.

关 键 词：蒙药 成分复方 乳房炎 模型小鼠 治疗作用 

分 类 号：S853.74[农业科学—临床兽医学] S858.237.26[农业科学—兽医学]