神经生长因子对糖皮质激素诱导大鼠海马神经元凋亡的保护作用  

Protective effect of nerve growth factor on glucocorticoid-induced apoptosis of primary cultured rat hippocampal neurons

作　　者：夏颖华[1] 孔晓冬[1] 雷平[1] 张释双[1] 张明义[1] 赵子龙[2] 葛歆瞳 

机构地区：[1]天津医科大学总医院保健医疗部天津市老年病学研究所,300052 [2]天津医科大学总医院神经外科天津市神经病学研究所天津市神经损伤变异与再生重点实验室教育部中枢神经系统创伤修复与再生重点实验室,300052

出　　处：《中国现代神经疾病杂志》2017年第3期209-213,共5页Chinese Journal of Contemporary Neurology and Neurosurgery

基　　金：天津市应用基础与前沿技术研究计划项目(项目编号:15JCYBJC50500);天津市卫生局科技基金资助项目(项目编号:2015KZ118);天津医科大学总医院青年孵育基金资助项目(项目编号:ZYYFY2015034)~~

摘　　要：目的探讨神经生长因子对糖皮质激素诱导的大鼠海马神经元凋亡的保护作用。方法体外分离原代培养18只新生Wister大鼠海马神经元,噻唑蓝法测定地塞米松诱导海马神经元凋亡的最低敏感剂量,观察不同质量浓度神经生长因子对地塞米松(0.10×10^(-6)mol/L)诱导海马神经元凋亡的保护作用。结果与阴性对照组相比,地塞米松Ⅰ组(10×10^(-6)mol/L)、Ⅱ组(1×10^(-6)mol/L)和Ⅲ组(0.10×10^(-6)mol/L)大鼠海马神经元活性均降低(P=0.000,0.000,0.000)。予不同质量浓度神经生长因子后,神经生长因子0.18 ng/ml组大鼠海马神经元活性低于阴性对照组(P=0.000)和阳性对照组(P=0.010),神经生长因子18 ng/ml组大鼠海马神经元活性高于阳性对照组(P=0.000)和神经生长因子0.18 ng/ml组(P=0.000)。结论糖皮质激素可以诱导体外培养的大鼠海马神经元凋亡,地塞米松0.10×10^(-6)mol/L是诱导海马神经元凋亡的最低敏感剂量,神经生长因子可以拮抗地塞米松诱导的大鼠海马神经元凋亡。.3Objective To observe the protective effect of nerve growth factor（NGF） on apoptosis of primary cultured rat hippocampal neurons which were induced by glucocorticoids. Methods The neurons isolated from the hippocampus of 18 neonatal Wister rats were cultured in vitro. Methyl thiazolyl tetrazolium（MTT） analysis was used to detect the lowest concentration of dexamethasone-induced hippocampal neuronal apoptosis,so as to explore the protective effect of different concentrations of NGF on0.10 × 10^-6mol/L dexamethasone-induced hippocampal neuronal apoptosis. Results Compared with negative control group,the activity of rat hippocampal neurons was reduced significantly in dexamethasoneⅠ（10 × 10^-6mol/L）,Ⅱ（1 × 10^-6mol/L） and Ⅲ（0.10 × 10^-6mol/L） groups（P = 0.000,0.000,0.000）. After different concentrations of NGF were given,the activity of hippocampal neurons in NGF 0.18 ng/ml group was significantly lower than negative control group（P = 0.000） and positive control group（P = 0.010）,whilethe activity of hippocampal neurons in NGF 18 ng/ml group was significantly higher than positive control group（P = 0.000） and NGF 0.18 ng/ml group（P = 0.000）. Conclusions Glucocorticoids can induce the apoptosis of in vitro cultured rat hippocampal neurons,and 0.10 × 10^-6mol/L dexamethasone is the lowest sensitive dose. NGF plays a role of blocking dexamethasone-induced apoptosis.

关 键 词：神经生长因子 糖皮质激素类 海马 细胞凋亡 细胞 培养的 疾病模型 动物 

分 类 号：R96[医药卫生—药理学]