扶正祛邪方对糖尿病肾病大鼠肾组织MCP-1的影响  

Effect of Fuzheng Quxie Prescription on Renal Tissue MCP-1 of Rats with Diabetic Nephropathy

作　　者：朱章志[1] 曾绘域 廖华君[2] 许帅[2] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广州中医药大学,广东广州510405

出　　处：《新中医》2017年第4期12-15,共4页New Chinese Medicine

摘　　要：目的:观察扶正祛邪方对糖尿病肾病大鼠肾组织单核细胞趋化蛋白-1(MCP-1)表达的影响,探讨扶正祛邪方对糖尿病肾病的保护作用及机制。方法:将80只SD大鼠随机分为正常组10只,造模组70只。造模组大鼠予腹腔内注射链脲佐菌素(STZ)进行造模,最后成功建立糖尿病肾病模型大鼠48只,按随机数字表进一步分为模型组、扶正祛邪方组、厄贝沙坦组,每组16只。扶正祛邪方组按16.38 g/(kg·d)的剂量灌胃,厄贝沙坦组按13.5 mg/(kg·d)的剂量灌胃,正常组及模型组灌服等量蒸馏水。给药8周后腹主动脉采血测空腹血糖,收集24 h尿液测尿蛋白,HE染色观察肾组织病理学改变,Western blot检测各组肾组织中MCP-1的表达。结果:治疗前,与正常组比较,模型组的空腹血糖、24 h尿蛋白、MCP-1蛋白显著升高,差异均有统计学意义(P<0.01),说明模型成立。治疗后,与模型组比较,扶正祛邪方组空腹血糖、24 h尿蛋白、MCP-1蛋白显著降低;厄贝沙坦组24 h尿蛋白、MCP-1显著降低,差异均有统计学意义(P<0.01)。治疗后,与治疗前比较,扶正祛邪方组空腹血糖、24 h尿蛋白显著降低;厄贝沙坦组24 h尿蛋白显著降低,差异均有统计学意义(P<0.01)。治疗后,与厄贝沙坦组比较,扶正祛邪方组空腹血糖降低的更明显,差异有统计学意义(P<0.01)。HE染色显示模型组大鼠肾小球体积增大,部分肾小球基底膜增厚,系膜基质增生,肾小管上皮细胞可见空泡样变性,扶正祛邪方组及厄贝沙坦组上述病理改变均明显轻于模型组。结论:扶正祛邪方能降低血糖,改善糖尿病肾病的病理学改变,能下调肾脏MCP-1的表达,起到减少尿蛋白排出,延缓DN进展的作用。Objective：To observe the effect of Fuzheng Quxie prescription on monocyte chemoattractant protein-1（MCP-1） form renal tissue of rats with diabetic nephropathy,and discuss its protection and mechanism for the treatment of diabetic nephropathy.Methods：Selected 80 SD rats,and divided them into normal group with 10 cases and modeling group with 70 cases.The modeling group was given intraperitoneal injection of streptozotocin（STZ） to create the models.Finally,48 rats with diabetic nephropathy were successfully modeled.Divided them into model group,Fuzheng Quxie prescription group and ibesartan group with 16 cases in each.Fuzheng Quxie prescription group was given gavage with 16.38 g/（kg·d）,ibesartan group was given gavage with 13.5 mg/（kg·d）,normal group and model group were given equivalent distilled water.After eight weeks of administration,detected fasting blood glucose by aortic blood collection,collected 24 h urine to detect urine protein,observed pathological changes of renal tissue with hematoxylin and eosin（HE） staining,detected expression of MCP-1 in renal tissue of each group with Western blot.Results：Before treatment,comparing with those in the normal group,the fasting blood glucose、24h urinary protein and MCP-1 in the model group were significantly increased（P〈0.01）,which suggested the model was successfully established.After treatment,comparing with those in the model group,fasting blood glucose、24h urine protein and MCP-1 of Fuzheng Quxie prescription group were significantly decreased,24 h urine protein and MCP-1 of ibesartan group were significantly decreased,the difference being significant（P 0.01）.After treatment,comparing with those before treatment,the fasting blood glucose and 24 h urinary protein in the Fuzheng Quxie prescription group were significantly increased,24 h urine protein of ibesartan group was significantly decreased,the difference being significant（P〈0.01）.After treatment,comparing with that of the ibesartan group,decrease of fasting bl

关 键 词：糖尿病肾病 扶正祛邪方 厄贝沙坦 单核细胞趋化蛋白-1(MCP-1) 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]