Raptor、Rictor和磷酸化Akt在日光性角化病、Bowen病及鳞状细胞癌中的表达  

Expression of Raptor,Rictor and p-Akt in actinic keratosis,Bowen's disease and squamous cell carcinoma

作　　者：周萌[1] 刘保国[2] 顾静[1] 苗国英[2] 李小静[2] 刘青[2] 

机构地区：[1]承德医学院研究生院,河北承德067000 [2]河北工程大学附属医院皮肤科,河北邯郸056002

出　　处：《中国麻风皮肤病杂志》2017年第3期132-136,共5页China Journal of Leprosy and Skin Diseases

基　　金：河北省政府资助临床优秀人才培训项目(编号:361037);邯郸市科技技术研究项目(编号:1523108076)

摘　　要：目的:检测Raptor、Rictor和磷酸化Akt(p-Akt)在日光性角化病、Bowen病和鳞状细胞癌中的表达。方法:采用免疫组化法检测Raptor、Rictor及p-Akt(Ser473)在20例正常皮肤、20例日光性角化病、20例Bowen病及40例鳞状细胞癌中的表达。结果:Raptor在鳞状细胞癌、Bowen病和日光性角化病中的阳性表达率分别为87.50%、70.00%和60.00%,均高于正常皮肤的25.00%(均P<0.05);其中低分化鳞状细胞癌中Raptor的阳性表达率为100%,高于高分化鳞状细胞癌的阳性表达率75%(P<0.05)。Rictor在鳞状细胞癌、Bowen病和日光性角化病中的阳性表达率分别为80.00%、70.00%及55.00%,均高于正常皮肤阳性表达率的20%(均P<0.05);p-Akt(Ser473)在鳞状细胞癌、Bowen病和日光性角化病中的阳性表达率分别为77.50%、65.00%及50.00%,而正常皮肤阳性表达率为0。鳞状细胞癌、Bowen病和日光性角化病中Rictor的阳性表达水平和p-Akt(Ser473)的阳性表达水平均呈正相关(均P<0.05)。结论:Raptor、Rictor和p-Akt(Ser473)的高表达可能与日光性角化病、Bowen病和鳞状细胞癌的发生发展有关。Objective： To detect the expression of Raptor,Rictor and p-Akt in the lesions of actinic keratosis（AK）,Bowen＇s disease（BD） and squamous cell carcinoma（SCC）. Methods： Immunohistochemistry was used to detect the expression of Raptor,Rictor,p-Akt（Ser473） in the tissue samples from the patients with AK,BD,SCC and normal human skin. Results： The positivity rates of Raptor in SCC,BD and AK were87.50%,70.00% and 60.00%,which was significantly higher than that in normal skin（Ps〈0.05）. The positivity rate was 100% in poorly differentiated squamous cell carcinoma and 75% in well differentiated squamous cell carcinomas,with a significant difference（P〈0. 05）. The positivity rates of Rictor in SCC,BD and AK were 80.00%,70.00% and 55.00%,which was significantly higher than that in normal skin（P〈0. 05）. The positivity rates of p-Akt（Ser473） in SCC,BD and AK were 77.50%,65.00% and 50.00%,while there was no positive staining in normal skin. There was a positive correlation between the expression level of Rictor and p-Akt（Ser473） in SCC,BD and AK（P〈0. 05）. Conclusion： The high expression of Raptor,Rictor and p-Akt（Ser473） may play a key role in the occurrence and development of AK,BD and SCC.

关 键 词：鳞状细胞癌 BOWEN病 日光性角化病 RAPTOR RICTOR 

分 类 号：R758.1[医药卫生—皮肤病学与性病学] R739.5[医药卫生—临床医学]