水曲柳WRKY40基因的克隆及表达模式分析  

Cloning and Expression Analysis of FmWRKY40 Gene in Fraxinus mandshurica

作　　者：刘颖[1] 何利明[1] 赵兴堂[1] 梁楠松[1] 李新宇[1] 梁雪[1] 詹亚光[1,2] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]林木遗传育种国家重点实验室,东北林业大学,哈尔滨150040

出　　处：《分子植物育种》2017年第3期833-838,共6页Molecular Plant Breeding

基　　金：国家基金(31270697);国家科技支撑项目(2012BAD21B0201)共同资助

摘　　要：从水曲柳中克隆一个WRKY基因,序列比对后命名为Fm WRKY40,基因编码区全长为936 bp,编码331个氨基酸(GenBank登录号:KU928310);含有一个WRKY保守结构域和一个C2H2锌指结构域,属于WRKY家族第Ⅱ类成员;分子进化分析表明,Fm WRKY40与芝麻WRKY40亲缘关系最近,蛋白相似性为73%;qRT-PCR表达模式分析表明:Fm WRKY40在干旱胁迫和ABA(脱落酸)胁迫下明显上调表达,该基因在水曲柳应答干旱和其他非生物胁迫中可能起重要的调控作用。We cloned a WRKY gene from Fraxinus mandshurica named as Fm WRKY40 after blast. The full length of coding region was 936 bp, which encoded 331 amino acids（GenBank accession number： KU928310）. The amino acid sequence involved a conserved WRKY domain and a zinc finger structure（C2H2）, which belonging to Group Ⅱ of WRKY family. Phylogenetic analysis showed that, Fm WRKY40 had closest relationship with sesame seeds WRKY40, the similarity of protein domain sequence was 73%. QRT-PCR expression pattern analysis showed that Fm WRKY40 significantly upregulated under the stress of drought and ABA（Abscisic acid）. This gene may play an important role in drought and other abiotic stresses response in Fraxinus mandshurica.

关 键 词：水曲柳 WRKY转录因子 实时荧光定量PCR 干旱胁迫 脱落酸 

分 类 号：S792.41[农业科学—林木遗传育种] Q943.2[农业科学—林学]