白刺花种子转录组分析  

Transcriptomics Analysis of Hard Seeds of Sophora viciifolia

作　　者：刘杰[1,2] 罗充[1] 孙威[1,2] 乙引[1,2] 

机构地区：[1]贵州师范大学生命科学学院,贵阳550001 [2]贵州省植物生理与发育调控重点实验室,贵阳550001

出　　处：《分子植物育种》2017年第3期867-874,共8页Molecular Plant Breeding

基　　金：贵州省植物生理与发育调控重点实验室建设;贵州省重点实验室建设项目(黔科合计Z字[2011]4005);教育部喀斯特山地生物多样性保护与可持续利用创新团队;长江学者和创新团队发展计划项目(IRT1227);贵州师范大学博士科研启动项目(11904-0514012);贵州省科技厅-贵州师范大学联合基金(黔科合LH字[2016]7210号)共同资助

摘　　要：为探索白刺花(Sophora viciifolia)硬实形成的相关机制,采用Illumina Hi Seq TM 2000高通量测序技术对白刺花种子转录组进行测序,利用Trinity软件将数据组装形成转录本,对所有转录本进行Nr(NCBI nonredundant protein sequences)、Nt(NCBI nucleotide sequences)、Pfam(protein family)、KOG/COG(eu Karyotic ortholog groups/clusters of orthologous groups)、Swiss-Prot(A manually annotated and reviewed protein sequence database)、KEGG(kyoto encyclopedia of genes and genomes)和GO(gene ontology)分类和功能注释、Pathway注释,并对种子形成的代谢通路中的相关基因进行了分析。转录组共获得了333 339 724条初始序列,总长为335 557 bp,初始序列组装获得序列片段的平均长度与N50值分别为282 bp和537 bp;与KOG功能注释、GO分类及KEGG代谢通路分析后,获得了44 840个GO功能注释、46 126个KOG功能注释以及89 494个PFAM注释:并从KEGG通路中找到有色氨酸代谢、半胱氨酸和甲硫氨酸的代谢途径的编码基因片段分别有66和37个。In order to understand the mechanism of hard seeds in Sophora viciifolia, the experiment used Illumina Hi SeqTM2000 high-throughput sequencing technology to sequence its transcriptome. Trinity soft was used to assemble the data to form transcripts. Based on the Nr（NCBI non-redundant protein sequences）, Nt（NCBI nucleotide sequences）, Pfam（protein family）, KOG/COG（eu Karyotic ortholog groups/clusters of orthologous groups）, SwissProt（A manually annotated and reviewed protein sequence database）, KEGG（kyoto encyclopedia of genes and genomes） and GO（gene ontology） databases, the experiment carried out functional annotation and classification, and the pathway annotation. The correlative genes in the hard seed forming process were analyzed. The results showed that333 339 724 reads were acquired and the total length was 335 557 bp. The mean length and N50 length were 282 bp and 537 bp, respectively. Among of them, 46 126 annotations was from KOG, 44 840 from GO and 89 494 from PFAM after functional annotation against these databases. From KEGG pathway, 66 and 37 unigenes were associated with the pathway of tryptophan metabolism, cysteine and methionine biosynthesis, respectively.

关 键 词：白刺花 转录组 硬实 高通量测序 

分 类 号：S793.9[农业科学—林木遗传育种] Q943.2[农业科学—林学]