河南粳稻抗稻瘟病基因Pi9、Pita和Piz-t的分子检测  

Molecular Detection of Rice Blast Resistance Gene Pi9, Pita and Piz-t in Henan Japonica Rice

作　　者：王生轩[1,2] 李俊周[1] 谢瑛[3] 李勇[3] 李梦琪[2] 曹炳武 赵全志[1] 

机构地区：[1]河南粮食作物协同创新中心,河南省水稻生物学重点实验室,水稻河南省工程实验室,河南农业大学,郑州450002 [2]河南省农业科学院,郑州450002 [3]信阳市农业局,信阳464000

出　　处：《分子植物育种》2017年第3期951-955,共5页Molecular Plant Breeding

基　　金：河南省重大科技专项(141100110600);河南省教育厅科学技术研究重点项目(14A210009);郑州市节水农业重点实验室建设项目(121PYFZX185);郑州市普通科技攻关计划项目(153PKJGG106)共同资助

摘　　要：为明确Pi9、Pita和Piz-t 3个抗稻瘟病主效基因在河南水稻新品系的分布状况。本研究对2015年河南省沿黄粳稻品种筛选试验的21份水稻新品系进行Pi9、Pita和Piz-t 3个抗稻瘟病基因的分子鉴定。结果表明,21个检测品系中有9个含有Pi9抗性等位基因,5个含有Pita抗性等位基因,11个含有Piz-t抗性等位基因;只有一个品系汴粳3号携带3个基因的抗性等位基因,菡科18、新丰1768、豫农粳13号、圣稻070、信粳1787、郑稻23和光灿8号7个材料携带两个基因的抗性等位基因。Pi9和Piz-t基因在河南水稻稻瘟病育种中利用较为广泛,Pita利用较少,今后河南水稻育种应加强广谱稻瘟病抗性基因的聚合育种和综合利用。In order to make clear the distribution of three rice blast resistance genes Pi9, Pita and Piz-t in Henan new rice lines, molecular detection using markers of Pi9, Pita and Piz-t in 21 new japonica rice lines of screening experiment of along the Yellow river in Henan province of 2015 were conducted in this study. The results showed that there were 9 lines containing Pi9 resistant alleles, 5 lines containing Pita resistant alleles, 11 lines containing Piz-t resistant alleles in the 21 tested lines. Only one line, Bianjing 3, contained resistant alleles of three genes.Seven lines, Hanke 18, Xinfeng 1768, Yunongjing 13, Shengdao 070, Xinjing 1787, Zhengdao 23, and Guangcan8, contained resistant alleles of two genes. Pi9 and Piz-t genes are widely used in rice blast breeding in Henan,while Pita is less. Pyramiding breeding and comprehensive utilization of broad-spectrum blast resistance gene should be strengthened in Henan rice breeding in future.

关 键 词：水稻 稻瘟病 Pi9 Pita Piz-t 分子检测 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]