右美托咪定预处理减轻H9C2细胞缺氧／复氧损伤  

Protective effect of dexmedetomidine preconditioning on H9C2 cells hypoxia/reoxygenation injury

作　　者：高金孟 孟晓文[1] 王丽娜[1] 杨宇帆[1] 嵇富海[1] 

机构地区：[1]苏州大学附属第一医院麻醉科,215000

出　　处：《国际麻醉学与复苏杂志》2017年第3期198-202,共5页International Journal of Anesthesiology and Resuscitation

基　　金：国家自然科学基金（81471835,81471889）

摘　　要：目的探讨右美托咪定（dexmedetomidine，Dex）的抗炎作用在抑制H9C2心肌细胞缺氧／复氧（hypoxia/reoxygenation，H／R）损伤中的作用。方法体外培养H9C2细胞，采用连二亚硫酸钠诱导H／R损伤模型。采用随机数字表法分为：正常对照组（C组），常规培养细胞；H／R组，4mmol／L连二亚硫酸钠处理缺氧1h，换正常培养基继续培养进行复氧12h；Dex组（D组），缺氧前经不同浓度Dex（0．1、1．0、10．0μmol／L）预处理1h，其余处理同H／R组。采用甲基噻唑蓝（3-[4，5-dimethylthiazol-2-y1]-2，5diphen），hetrazoliumbromide，MTT）法检测细胞存活率，2，4-二硝基苯肼显色法检测培养基乳酸脱氢酶（lactate dehydrogenase，LDH）活性，倒置显微镜观察H9C2心肌细胞形态，RT-PCR检测TNF啾、IL-6、IL-1βmRNA的表达水平。结果与c组比较，H／R组细胞存活率低至（44±12）％（P〈0．01），LDH活性显著升高（P〈0．01）；与H／R组比较，Dex可不同程度地提高H9C2细胞存活率，降低LDH活性，且1．0μmol／LDex预处理可将细胞存活率提高至（75±7）％（P〈0．01），显著降低LDH活性（P〈0．05）。倒置显微镜观察到与c组比较，H／R组细胞排列不规则、细胞坏死、细胞膜折光率降低，Dex可以改善H9C2细胞的形态学改变。与C组比较，H／R组TNF-α、IL-6、IL-1βmRNA的表达水平显著升高（P〈0．05）；与H／R组比较，D组TNF-α、IL-6、IL-1βmRNA的表达水平显著降低（P〈0．05）。结论Dex预处理可减轻H9C2细胞H／R损伤，其机制可能与抑制炎症反应有关。Objective To explore the anti-inilammatory effect of dexmedetomidine （Dex） in attenuating hypoxia/reoxgenation （H/R） injury of H9C2 cells. Methods Rat cardiomyocyte cell line H9C2 cells were cultured in vitro, and Na2S2O4 was used to establish the hypoxia and reoxygenation injury model on H9C2 cells. Cultured H9C2 cells were divided into three groups： normal control group （group C）, the cells were cultured as usual, group H/R, the cells were subjected to l h hypoxia induced by 4 mmol/L Na2S2O4, then followed by 12 h reoxygenation by replaced the normal media, Dex preconditioning group （group D）, Dex at various concentrations（0.1, 1.0, 1 0.0μxmol/L） was performed prior to Na2S2O4-induced H/R injury, the rest of the steps are essentially the same as the group H/R. The cell survival rate 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide （MTT） and lactate dehydrogenase（LDH） activity were detected ＇after treatment, the cellular morphology was observed by inverted miscroscope, the TNF-α, IL-6, IL-1β mRNA expression were detected by reverse transeription-polymerase chain reaction （RT-PCR）. Results Compared with the group C, the cell survival of group H/R decreased to （44±12）%（P〈0.01）, LDH activity increased significantly （P〈0.01）, compared with the group H/R, the cell survival rate and LDH activity of the group D were improved, Dex at 1μmol/L concentration preconditioning significantly restored cell viability to （75±7）%（P〈0.05） and reduced the activity of LDH significantly （P〈0.05）. The condition of cell necrosis, irregular cell arrangement, and the decline of cell membrane refractive index caused by H/R injury were partly reversed by Dex. Compared with the group C, the TNF-α, IL-6, IL-1β mRNA expression level of the group D and the group H/R increased significantly. Compared with group H/R, the TNF-α, IL-6, IL-1β mRNA expression levels of group D decreased significantly（P〈0.05）. Conclusions Dex preconditioning attenuates hy

关 键 词：右美托咪定 预处理 缺氧/复氧损伤 炎症反应 

分 类 号：R614[医药卫生—麻醉学]