夏枯草多糖及凝胶抗单纯疱疹病毒的药效学研究  

A Pharmacodynamic Study on the Anti-Herpes Simplex Virus(HSV) Activity of the Polysaccharides and Gels from Prunella vulgaris L.

作　　者：蔡双璠 杨扬[3] 吴蓉[1,2] 杨以阜[3] 谭红胜[1,2] 

机构地区：[1]上海中医药大学中药学院,上海201203 [2]中药创新药物研发上海高校工程研究中心,上海201203 [3]上海中医药大学科技实验中心,上海201203

出　　处：《世界科学技术-中医药现代化》2017年第2期247-253,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部"重大新药创制"科技重大专项"十二五"计划(2013ZX09103002-020):夏枯草多糖抗疱疹病毒候选药物研究;负责人:徐宏喜

摘　　要：目的:探究夏枯草多糖及凝胶在体外和体内条件下的抗单纯疱疹病毒作用,为抗疱疹病毒新药研究提供科学依据。方法:本研究采用空斑减数法检测夏枯草多糖的体外抗单纯疱疹病毒(Herpes Simplex Virus,HSV)活性;同时,建立HSV-1(皮肤)和HSV-2(外阴部)病毒感染豚鼠模型,以病灶病变情况、丘疱疹数、典型病变评分结果以及病灶组织中HSV-1和HSV-2病毒的DNA拷贝数等为指标,对夏枯草多糖凝胶的体内抗HSV活性进行评价。结果:夏枯草多糖在体外实验中能够抑制HSV-1和HSV-2的活性;夏枯草多糖凝胶能够削弱HSV-1和HSV-2病毒感染豚鼠的病灶病变,发挥抗单纯疱疹病毒活性。结论:夏枯草多糖及凝胶在体外和体内实验中均具有抗单纯疱疹病毒的活性,具有开发成为抗单纯疱疹病毒药物的潜在价值。The aim of this study is to explore the anti-HSV activity of P. vulgaris polysaccharides and gels in vitro and in vivo with the provision of scientific evidence for the further research of anti-HSV new drugs. Plaque reduction assay was adopted to determine the IC50 value of P.vulgaris polysaccharides on HSV-1 and HSV-2 in vitro. In addition, HSV-1（skin） and HSV-2（vulva） infected guinea-pig models were established for assessing the anti-HSV activity of polysaccharides and gels in vivo, and infected lesion degree, number of papulovesicle, typical lesion score and the DNA copy numbers of HSV-1 and HSV-2 viruses in the lesion tissue were taken as the indexes. It was found that the activities of HSV-1 and HSV-2 viruses was inhibited by P. vulgaris polysaccharides in vitro, while HSV induced skin lesions in the guineapigs were ameliorated by P. vulgaris gel, exerting an anti-HSV action. In conclusion, it was demonstrated that P. vulgaris polysaccharides and gels performed an anti-HSV action both in vitro and in vivo with the hidden value of developing antiHSV agents.

关 键 词：夏枯草 抗单纯疱疹病毒 多糖 凝胶 

分 类 号：R285.5[医药卫生—中药学]