黄芩苷对人成纤维细胞样滑膜细胞(类风湿关节炎)的影响研究  

Effects of Baicalin on human fibroblast like synoviocytes( rheumatoid arthritis)

作　　者：王宏志[1] 葛存兴[1] 王广志[1] 赵晶[1] 姜楠[1] 

机构地区：[1]哈尔滨医科大学附属第五医院检验科,黑龙江大庆163316

出　　处：《齐齐哈尔医学院学报》2017年第2期129-131,共3页Journal of Qiqihar Medical University

基　　金：黑龙江省教育厅科学技术研究项目(12541523)

摘　　要：目的探讨黄芩苷对人成纤维细胞样滑膜细胞(类风湿关节炎)(HFLS-RA)分泌IL-1β、TNF-a水平和NF-κB信号转导通路的影响,为黄芩苷应用于类风湿关节炎临床治疗提供实验依据。方法将购买的HFLS-RA细胞进行传代培养,选用4-7代HFLS-RA进行细胞增殖试验(MTT)和药物实验研究。黄芩苷不同浓度组:HFLS-RA无血清的基础培养液中加入不同浓度的黄芩苷(10、20、30μm)及MTX 10μg/ml孵育24小时。对照组:HFLS-RA无血清基础培养液不加任何药物培养24小时。ELISA定量检测细胞培养上清液中TNF-α和IL-1β水平,western blot检测滑膜细胞中NF-κB p65、phospho-NF-κB p65(Ser536)和Acetyl-NF-κB p65(Lys310)蛋白表达。结果黄芩苷能明显降低细胞培养上清液中TNF-α、IL-1β含量,抑制滑膜细胞中NF-κB p65、phospho-NF-κB p65(Ser536)和Acetyl-NF-κB p65(Lys310)蛋白水平表达。结论黄芩苷抑制人成纤维细胞样滑膜细胞(类风湿关节炎)(HFLS-RA)可能是通过抑制炎性细胞因子产生和NF-κB信号转导途径而发挥治疗作用的。Objective To investigate the effect of Baicalin on the cellular secretion of IL-1 beta, TNF- alpha and NF-nuclear factor kappa B signal transduction pathway by the Human Fibroblast-Like Synoviocytes- Rheumatoid Arthritis （ HFLS-RA ）, and provide experimental evidence for clinical treatment of rheumatoid arthritis.Methods HFLS-RA cells were purchased for subculture, the use of HFLS-RA generation 4 - 7 cell proliferation test （MTT） and drug experimental study. Baicalin of different concentration groups： HFLS-RA serum-free culture with different concentrations of Baicalin in solution （ 10, 20, 30 M ） and MTX 10 g/ml incubated for 24 hours. Control group： HFLS-RA serum free medium without any drugs for 24 hours. Quantitative detection of TNF-alpha and IL-1 beta in cell culture supernatant by ELISA, The expression of NF-κB p65, phospho-NF-κB p65 （Ser536） and Acetyl-NF-KB p65 （Lys310） in synovial cells was detected with Western blot. Results Baicalin significantly reduced the contents of TNF-alpha and IL-1 beta in the cell culture supernatant, inhibit the expression of NF-κBp65, phospho-NF-κBp65 （Ser536） and Acetyl-NF-κBp65 （Lys310） protein in synovial cells.Conclusions Baicalin inhibits HFLS-RA may be through the inhibition of inflammatory cytokine production and NF-NF kappa B signal transduction pathway to play a therapeutic role.

关 键 词：黄芩苷 类风湿关节炎 人成纤维细胞样滑膜细胞(类风湿关节炎)(HFLS-RA) NF-ΚB 

分 类 号：R593.22[医药卫生—内科学]