Production of rhamnolipids-producing enzymes of Pseudomonas in E. coli and structural characterization  

作　　者：Kata Kiss Wei Ting Ng Qingxin Li 

机构地区：[1]Institute of Chemical & Engineering Sciences, Agency for Science, Technology and Research, Jurong Island 627833, Singapore

出　　处：《Frontiers of Chemical Science and Engineering》2017年第1期133-138,共6页化学科学与工程前沿（英文版）

摘　　要：Rhamnolipids are a class ofbiosurfactants that have a great potential to be used in industries. Five proteins/enzymes, namely RhlA, RhlB, RhlC, RhlG and RhlI, are critical for the production of rhamnolipids in Pseudomonas aeruginosa. Four of the 5 proteins except RhlC were successfully over-expressed in E. coli and three of them （RhlA, RhlB and RhlI） were purified and obtained in milligram quantities. The purified proteins were shown to be folded in solution. Homology models were built for RhlA, RhlB and RhlI. These results lay a basis for further structural and functional characterization of these proteins in vitro to favor the construction of super strains for rhamnolipids production.Rhamnolipids are a class ofbiosurfactants that have a great potential to be used in industries. Five proteins/enzymes, namely RhlA, RhlB, RhlC, RhlG and RhlI, are critical for the production of rhamnolipids in Pseudomonas aeruginosa. Four of the 5 proteins except RhlC were successfully over-expressed in E. coli and three of them （RhlA, RhlB and RhlI） were purified and obtained in milligram quantities. The purified proteins were shown to be folded in solution. Homology models were built for RhlA, RhlB and RhlI. These results lay a basis for further structural and functional characterization of these proteins in vitro to favor the construction of super strains for rhamnolipids production.

关 键 词：RHAMNOLIPIDS PSEUDOMONAS RhlA RhlB RhlI protein folding 

分 类 号：Q939.112[生物学—微生物学] Q78