应用拉曼成像技术研究疟原虫感染红细胞形态  

In situ fast Raman mapping of Plasmodium falciparum infected red blood cells

作　　者：董瑞玲[1,2] 刘铮 张琦慧[3] 易品[4] 朱玉兰[1,2] 古莉冰 孙杰[1,2] 何建安[1,2] 顾大勇[1,2] 

机构地区：[1]深圳国际旅行卫生保健中心,广东深圳518033 [2]深圳市检验检疫科学研究院 [3]满洲里国际旅行卫生保健中心 [4]广州医科大学附属第五医院

出　　处：《中国国境卫生检疫杂志》2017年第1期15-17,共3页Chinese Journal of Frontier Health and Quarantine

基　　金：国家质检总局科技计划项目(2016IK242;2015IK257;2014IK054);深圳出入境检验检疫局科技计划项目(SZ2015104);深圳市科技研发基金(CXZZ20150504163004399;JCYJ20150330102720128);广东省科技计划项目(2016A020219005)

摘　　要：目的探讨拉曼成像技术在疟疾检测中的应用效果。方法将体外培养的疟原虫感染的红细胞制成均匀的血涂片,采用Nano Photon Raman-11激光成像拉曼显微镜对血涂片进行扫描,获取拉曼光谱,设置参照峰1 372 cm^(-1)并以此峰强度进行拉曼成像。结果以1 372 cm^(-1)为基准的拉曼成像图中,可清晰观察到疟原虫感染红细胞的异常形态,感染红细胞拉曼成像图亮点与边缘部分、正常红细胞的拉曼光谱在1 372 cm^(-1)拉曼位移有显著峰强差别。结论拉曼成像技术可快速、准确地检测到疟原虫感染的红细胞,在疟疾研究中具有重要价值。Objective To investigate the application of Raman imaging system in the detection of malaria. Methods Plasmodium falciparum infected red blood cells （iRBCs） in the blood smear were scanned by NanoPhoton laser Raman fast imaging system. The Raman spectra of the iRBCs were obtained. Raman mapping was set according to the Raman shift of 1372cm^-1. Results The Raman spectra of brightened dot and margin edge from the iRBC had significant difference band intensity at 1 372cm^-1 Raman shift. The Raman mapping made by 1 372cm^-1 Raman shift showed abnormal form of iRBCs. Conclusion Raman imaging system had good resolution in the detection of malaria,and had important value in malaria research.

关 键 词：疟疾 红细胞 拉曼成像 

分 类 号：R382.31[医药卫生—医学寄生虫学] R318.51[医药卫生—基础医学]