虾血20-羟基蜕皮酮对瘢痕成纤维细胞增殖与Smad3 mRNA表达的影响  

The effects of 20-Hydroxyecdysone on the proliferation and Smad3 mRNA expression of hypertrophic scar fibroblasts in vitro

作　　者：刘双 赖银璇 李南 吴志贤 王丹纯 潘紫凤 陈嵘祎 

机构地区：[1]广东医科大学附属医院皮肤科,广东湛江524023 [2]广东医科大学附属医院健康管理中心,广东湛江524023 [3]广东医科大学附属医院整形外科,广东湛江524023 [4]广东医科大学第一临床学院,广东湛江524023

出　　处：《广东医学院学报》2016年第6期589-592,共4页Journal of Guangdong Medical College

基　　金：国家级大学生创新创业训练计划项目(No.201310571001);广东省大学生创新创业训练计划项目(No.1057113001)

摘　　要：目的探讨20-羟基蜕皮酮对增生性瘢痕成纤维细胞增殖与Smad3 mRNA表达的影响。方法以不同质量浓度(0.05、0.10、0.25 mg/L)20-羟基蜕皮酮分别处理体外培养的人瘢痕成纤维细胞(分别设为B、C、D组)24 h,同时加同体积无水乙醇溶剂作为对照(设为A组)。分别选用CCK8法、实时荧光定量PCR检测4组成纤维细胞的增殖情况及瘢痕增生相关Smad3 mRNA的表达。结果 CCK8法检测显示作用24 h后,4组的吸光度均数差异有统计学意义(P<0.01),其中A组最高,B组次之,C组较低,D组最低,两两比较差异均有统计学意义(均P<0.05或0.01)。实时荧光定量PCR结果显示20-羟基蜕皮酮可使瘢痕增生相关Smad3 mRNA的表达显著下调(P<0.01)。B、C和D组Smad3 mRNA表达均低于A组(均P<0.01)。结论 20-羟基蜕皮酮能抑制增生性瘢痕成纤维细胞的增殖与瘢痕增生相关Smad3 mRNA的表达。Objective To explore the effects of 20-Hydroxyecdysone on the proliferation and Smad3 mRNA expression of cultured human hypertrophic scar fibroblasts. Methods Human hypertrophic scar fibroblasts were cultured and stimulated by 20-Hydroxyecdysone with concentration 0.05, 0.10, 0.25 mg/L respectively and the control group is anhydrous ethanol. The cellular proliferation was measured with the cck8 staining method. The expression of Smad3 mRNA were evaluated by qRTPCR. Results There was significantly difference between experimental group and control group in absorbance（0.05 mg/L group P〈0.05, 0.10 mg/L, 0.25 mg/L group P〈0.01）, compared with control group, 20-Hydroxyecdysone can markedly inhibit the proliferation of cultured human hypertrophic scar fibroblasts. 20-Hydroxyecdysone also down-regulates expression of Smad3 mRNA（0.05 mg/L group P〈0.05, 0.1 mg/L, 0.25 mg/L group P〈0.01）. Conclusion 20-Hydroxyecdysone can inhibit the proliferation and Smad3 mRNA expression of hypertrophic scar fibroblasts and may become an effective drug for treatment of hypertrophic scar.

关 键 词：20-羟基蜕皮酮 增生性瘢痕 增殖 SMAD3 MRNA 

分 类 号：R282.77[医药卫生—中药学] R619[医药卫生—中医学]