粳稻资源热粳35遗传图谱构建与耐热QTL分析  

Construction of a Genetic Linkage Map and QTL Location for Heat Tolerance in Japonica Rice Resources Rejing35

作　　者：郑剑[1] 李兴星[1] 苏华英[1] 周军杰[1] 秦小健[1] 南文斌 杨永清[1] 张汉马 李贤勇[2] 梁永书[1,3] 

机构地区：[1]重庆师范大学,重庆401331 [2]重庆市农业科学院水稻所,重庆400060 [3]水稻生物学国家重点实验室,浙江杭州310006

出　　处：《核农学报》2017年第5期844-851,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家973前期专项(2014CB160306);重庆市自然科学基金(Cstc2014jcyj A80003);重庆市教委项目(KJ1703058)

摘　　要：为了揭示耐热粳稻资源热粳35的遗传特性,以热粳35/协B F2群体为研究材料,构建了包含140个SSR标记的分子图谱;借助构图分子数据分析标记基因型的分离情况,并进行耐热性QTL分析。结果表明,该图谱覆盖基因组全长2 157.7 c M,标记间平均图距15.4 c M,45个(28.5%)标记极显著偏向亲本或杂合子(P<0.01)。在第1、第3、第4、第6、第7和第8号染色体上发现9个偏分离热点区域。其中,有4个偏分离热点区域可能与孢子体或配子体选择有关,在第4、第8和第12号染色体上共检测到3个耐热性QTL。本研究结果为耐热粳稻资源的遗传机理解析和新品种选育提供了参考信息。To elucidate the genetic mechanism of heat tolerant （HT） variety Rejing35, a microsatellite-based linkage map of Rejing35 was designed using 140 SSR markers and a F2 population comprising 226 plants. The F2 population was derived from the crossover between Rejing35 and XieB. The molecular data was used to analyze the genotype segregation and detect QTL underlying HT, respectively. The results indicated that the map has spanned 2 157.7 cM with an average marker distance of 15.4 cM. Altogether 45 markers （28.5%） significantly showed distortion towards biparents or heterozygote （P〈0.01）. There were 9 clustered distortion regions distributed on the 1^st, 3^rd, 4^th, 6^th,7^th and 8^th chromosomes, respectively. Among them, 4 distortion regions may be caused by gametophyte and zygote. A total of 3 QTLs controlling HT were detected on the 4^th, 8^th, and 12^th chromosomes, respectively. These results will provide valuable insights into the molecular mechanism underlying HT and a novel gene resource for HT rice variety breeding.

关 键 词：水稻 SSR 偏分离 耐热性QTL 

分 类 号：S511.22[农业科学—作物学]