厚垣普可尼亚菌航天诱变的生物学效应  

Biological Effect of Space Mutagenesis Pochonia chlamydosporia

作　　者：孟繁丽[1] 汪来发[1] 王曦茁[1] 薛寒[1] 苟大平[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所/国家林业局森林保护学重点实验室,北京100091

出　　处：《核农学报》2017年第6期1037-1045,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家林业局林业科学技术推广项目([2016]09);国家微生物资源平台(NIMR2017-7)

摘　　要：为探讨厚垣普可尼亚菌航天诱变的生物学效应,对筛选得到的29个厚垣普可尼亚菌航天诱变菌株进行形态、色素、孢子形态、菌落生长速度、菌丝干重、产孢量和致病力等生物学特性检测,并测定各航天诱变菌株的耐盐性以及对苯菌灵的抗性。结果表明,厚垣普可尼亚菌航天诱变菌株各项生物学特性与原始菌株存在差异。航天诱变后的菌株菌落形态和色素与原始菌株差异明显;菌落生长速度和菌丝干重的负突变率均高于正突变率;产孢量的正突变率高于负突变率;菌株Pc-m-4、Pc-m-6、Pc-m-10、Pcm-15和Pc-m-123对南方根结线虫卵的寄生率分别为92.33%、93.67%、91.67%、90.67%和90.33%,显著高于原始菌株的寄生率(81.00%),其中,菌株Pc-m-6具有较好的苯菌灵抗性,菌株Pc-m-10对盐具有较好的耐受性。航天诱变处理对厚垣普可尼亚菌影响显著,其诱变后的优良菌株具有防治南方根结线虫的潜力。To find the biological effect of space mutagenesis on P. chlamydosporia. Totally 29 strains isolated from the colonies of survival space-induced strains were tested not only for their biological characteristics,such as morphology,colony growing rate,dry weight of mycelium,spore production,and the parasitism to the eggs of Meloidogyne incognita,but also for salt tolerance and benomyl resistance. The results showed that the biological characteristics of space-induced strains of P. chlamydosporia were significantly different from the original strain. For space-induced strains,the colony growing rate and dry weight of mycelium showed higher negative mutation rates,the spore production showed greater positive mutation rates,and the pathogenicity against the eggs of M. incognita was more great,for instance,the improved parasitic rates of Pc-m-4,Pc-m-6,Pc-m-10,Pc-m-15 and Pc-m-123 were 92. 33%,93. 67%,91. 67%,90. 67% and 90. 33%,respectively,higher than the parasitic rate of original strain（ 81. 00%）,besides,Pc-m-6 had a good resistance to the benomyl and Pc-m-10 was able to survive in the high salinity condition. The influence of space mutagenesis on P. chlamydosporia was significant,and the superior strains obtained showed potential in biological control of M. incognita.

关 键 词：厚垣普可尼亚菌 航天诱变 生物学特性 抗逆性 生物防治 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]