3种常规消毒方法对磺胺类抗性基因削减效果的比较  

Reducing Effect of Three Disinfection Technologies for Sulfonamides Resistance Genes

作　　者：郑吉[1] 周振超[1] 陈芳[2] 陈涛[1] 魏媛媛[1] 韩玥[1] 陈红[1] 

机构地区：[1]浙江大学环境与资源学院环境工程研究所,杭州310058 [2]杭州市环境监测中心站,杭州310007

出　　处：《环境科学》2017年第4期1497-1505,共9页Environmental Science

基　　金：国家水体污染控制与治理科技重大专项(2014ZX07101-012);国家自然科学基金项目(21277117;21210008)

摘　　要：近年来,抗生素抗性基因(antibiotic resistance genes,ARGs)对人类和环境的潜在风险引起了广泛关注.本研究从1家城市污水处理厂二级出水中分离出两株磺胺类抗性细菌,控制浓度投加到灭菌后的出水中,分别进行氯消毒、紫外消毒、臭氧消毒处理,采用菌落计数法、q PCR法对消毒后目标细菌及磺胺类抗性基因(sulⅠ、sulⅡ)进行分析比较.同时采用"消毒+DNase I酶"实验研究磺胺类抗性基因在各消毒过程中的行为特征.结果表明,3种消毒均有效削减细菌浓度,但对抗性基因削减程度不高.结合"消毒+DNase I酶"实验分析,氯消毒削减磺胺类抗性基因与微生物量的降低有关;紫外消毒能直接破坏抗性基因;而臭氧消毒在大量削减细菌量的同时,抗性基因进入到胞外环境中,其潜在环境风险不容忽视.Antibiotic resistance genes（ARGs） in the environment have gradually engendered extensive concern recently due to the harm caused to environment and human beings.In this study,two sulfonamides resistant bacteria isolated from secondary effluents of a municipal wastewater treatment plant were used to compare the efficacy of three different disinfection processes（chlorination,ultraviolet and ozone） in reducing sulfonamides resistance pollution using serial dilution plate counting method and q PCR.The behavior characteristics of sulfonamides resistance genes during the disinfection process were elucidated using disinfection ＋ DNaseⅠ treatment.The results showed that all three disinfection techniques could reduce the concentration of target bacteria,but the abundance of sulfonamides resistance genes was still at a high level.Furthermore,chlorination disinfection might reduce sulfonamides resistance genes by reducing microbial biomass.Ultraviolet disinfection could damage genes directly.However,during ozone disinfection,sulfonamides resistance genes would escape to environment,and its potential environmental risks could not be ignored.

关 键 词：消毒 磺胺类 抗生素抗性基因 DNaseⅠ酶 荧光定量PCR 

分 类 号：X703.1[环境科学与工程—环境工程]