天门冬酰胺酶羟丙基-β-环糊精脂质体的稳定性及相关机制的初步研究  

Preliminary study on the stability and related mechanism of L-asparaginase loaded in hydroxypropyl-β-cyclodextrin liposomes

作　　者：万胜利[1] 李瑶[1] 何丹[1] 晏子俊[1] 张景勍[1] 

机构地区：[1]重庆医科大学重庆药物高校工程研究中心,重庆400016

出　　处：《华西药学杂志》2017年第2期157-159,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:30973645)

摘　　要：目的考察天门冬酰胺酶(L-Asp)羟丙基-β-环糊精脂质体(AHL)的稳定性及相关机制。方法采用逆相蒸发法制备AHL,对其低温放置稳定性、热稳定性、酸碱稳定性、血浆稳定性、抗胰蛋白酶水解能力及相关机制进行初步研究。结果 AHL体外稳定性都优于L-Asp,能阻止L-Asp构象的改变。结论 AHL能提高L-Asp的稳定性。OBJECTIVE To investigate the stability and related mechanism of L - asparaginase （L - Asp ） loaded in hydroxypropyl- β- cyclodextrin liposomes （AHL）. METHODS The reverse evaporation method was used to prepare AHL. The temperature stability, acid - base stability, plasma stability, proteolytic stability, and related mechanism of AHL were investigated. RESULTS AHL could improve the in vitro stability of L - Asp. AHL might inhibit the conformational changes of L - Asp. CONCLU- SION AHL can improve the stabilities of L - Asp.

关 键 词：天门冬酰胺酶 羟丙基-β-环糊精脂质体 稳定性 逆相蒸发法 机制 

分 类 号：R94[医药卫生—药剂学]