穿膜肽TAT修饰的自噬诱导肽Beclin 1抗肿瘤的初步研究  

Preliminary study on the anti-tumor effect of cell-penetrating peptide TAT decorated autophagy inducing peptide Beclin 1

作　　者：余倩雯[1] 胡关莲 王蛘 高会乐[1] 何勤[1] 

机构地区：[1]四川大学华西药学院,成都四川610041

出　　处：《华西药学杂志》2017年第2期163-166,共4页West China Journal of Pharmaceutical Sciences

摘　　要：目的研究自噬诱导肽Beclin 1及其经穿膜肽TAT修饰后所得TAT-Beclin 1对乳腺癌的体外自噬诱导作用及体内外疗效。方法采用透射电子显微镜、eGFP-LC3细胞转染技术和蛋白免疫印迹法综合对比评价Beclin 1和TAT-Beclin 1对4T1细胞自噬流的影响,通过体外细胞毒性和体内抑瘤实验评价其抗肿瘤疗效。结果 TAT-Beclin 1能显著提高Beclin 1在4T1细胞上的自噬诱导能力;TAT-Beclin 1在体内具有一定的抗4T1肿瘤能力。结论 TAT-Beclin 1较Beclin 1能更有效地诱导自噬并发挥治疗乳腺癌的效果。OBJECTIVE To investigate the therapeutic effect for breast cancer of cell - penetrating peptide TAT decorated autophagy inducing peptide Beclin 1 in comparison with autophagy inducing peptide Beelin 1 in vivo and in vitro. METHODS The effects on autophagy flux of 4T1 cells of Beclin 1 and TAT - Beclin 1 were evaluated via comprehensive methods including TEM, eGFP - LC3 cell transfection technology and western blotting. Additionally,the anti - tumor effects of Beclin 1 and TAT - Beclin 1 were investigated by cell cytotoxicity in vitro and tumor growth inhibition experiments in vivo. RESULTS TAT - Beclin 1 significantly increased the ability of inducing autophagy on 4T1 cells ; TAT - Beelin 1 demonstrated toxic effect under the normal condition and facilitated the growth under serum free condition. TAT - Beclin 1 possessed the ability of inhibiting the growth of breast cancer. CONCLUSION TAT - Beclin 1 possessed stronger ability of inducing autophagy than Beclin 1, thereby improving the therapeutic effect for breast cancer.

关 键 词：TAT-Beclin 1 TAT 自噬诱导肽 穿膜肽 自噬流 4T1细胞 凋亡 乳腺癌 

分 类 号：R96[医药卫生—药理学]