芍药苷对大鼠急性胰腺炎合并肺损伤的保护作用及对核因子-κB信号传导通路的影响  

Protective effect of paeoniflori on rats with acute pancreatitis with lung injury and its effect on nuclear factor-κB signaling pathway

作　　者：丁月超[1] 马超[1] 余伟[1] 王谦[1] 蒙博[1] 韩风[1] 黄涛[1] 

机构地区：[1]郑州大学附属肿瘤医院(河南省肿瘤医院)肝胆胰外科,450008

出　　处：《中华实验外科杂志》2017年第3期385-387,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察芍药苷对大鼠急性胰腺炎（SAP）合并肺损伤的保护作用及对核因子-κB（NF-κB）信号传导通路的影响.方法 将大鼠随机分为SAP组、假手术组和芍药苷组,每组26只.建立大鼠SAP模型,分别于建模后6、12h采用酶联免疫吸附试验法检测各组大鼠血清淀粉酶（AMY）、相关炎性因子[白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α（TNF-α）]、肺组织髓过氧化物酶（MPO）的表达水平,采用实时荧光定量聚合酶链反应（FQ-PCR）法检测肺组织NF-κB的mRNA表达水平,并比较肺组织的湿/干重比值变化及胰腺、肺的组织病理学改变.结果 SAP组和芍药苷组术后6h大鼠血AMY[（4267±1459）、（2681±822）比（1482±5240）U/L]、肺MPO[（5.47±1.28）、（4.39±0.57）比（2.17±0.37）U/g]、肺组织的湿/干重比值（5.13±0.62、4.54±0.34比4.41±0.21）,显著高于假手术组,且术后12h差异更加明显.建模后12h胰腺组织病理评分[（7.44±1.57）比（11.30±2.27）分,P=0.021]、肺组织病理评分[（0.83±0.82）比（1.47±1.31）分,P=0.029]、血清TNF-α[（83.44±15.28）比（120.05±25.88）ng/L,P=0.042],IL-1β[（46.33±10.03）比（84.75±17.83）ng/L,P=0.036],IL-6[（72.77±18.51）比（92.81±19.92）ng/L,P=0.046]蛋白表达水平及NF-κB mRNA芍药苷组显著低于SAP组（P=0.002）.结论 芍药苷可能通过抑制NF-κB信号通路降低相关炎性因子IL-1β、IL-6、TNF-α的释放,从而达到对SAP合并肺损伤的保护作用.Objective To investigate the protective effect of paeoniflorin on acute pancreatitis with lung injury and its effect on nuclear factor-κB （NF-κB） signaling pathway.Methods Rats were randomly divided into 3 groups （n=26 each）： the sham operation group, severe acute pancreatitis （SAP） group, and the paeoniflorin group.After the model of ASP was established, the serum amylase （AMY）, interleukin （IL）-1β, IL-6 and tumor necrosis factor-α （TNF-α） expression levels were detected by enzyme linked immunosorbent assay （ELISA）.The NF-κB mRNA expression level was detected by real-time fluorescent quantitative polymerase chain reaction（FQ-PCR）, and the wet/dry ratio and pathological changes of lung tissue were measured and compared.Results The expression level of AMY [（4 267±1 459）, （2 681±822） vs.（1 482±5 240） U/L] in peripheral blood, MPO [（5.47±1.28）, （4.39±0.57） vs.（2.17±0.37） U/g] in the lung, and the wet/dry ratio （5.13±0.62, 4.54±0.34 vs.4.41±0.21） in the lung tissue in SAP group and the paeoniflorin group were significantly higher than in the sham operation group at 6 h after the operation, and the changes were even more significant in 12 h sub-groups.Pathological score of pancrea tissue （7.44±1.57 vs.11.30±2.27, P=0.021） and lung tissue （0.83±0.82 vs.1.47±1.31, P=0.029）, aortic blood TNF-α [（83.44±15.28） vs.（120.05±25.88） ng/L, P=0.042], IL-1β [（46.33±10.03） vs.（84.75±17.83） ng/L, P=0.036], IL-6 [（72.77±18.51） vs.（92.81±19.92） ng/L, P=0.046] protein levels and NF-κB mRNA in paeoniflorin group were significantly lower than in SAP group 12 h after the operation （P=0.002）.Conclusion Peoniflorin may have the positive protection on lung injury in SAP through inhibition of the NF-κB signaling pathway and thus reducing the release of inflammatory factors such as IL-1β, IL-6 and TNF-α.

关 键 词：芍药苷 急性胰腺炎 肺损伤 炎性反应 

分 类 号：R285.5[医药卫生—中药学]