miR-124a和miR-146a基因多态性与中国人群代谢综合征的相关性研究  

Association analysis between single nucleotide polymorphism of miR-124a,miR-146a and metabolic syndrome in Chinese Han populaticm

作　　者：李奕平[1] 杨曼[1] 熊煜欣[1] 史荔[2] 李显丽[1] 鲁帅尧[2] 王晓苓[1] 姚宇峰[2] 杨莹[1] LI Yi-ping YANG Man XIONG Yu-xin et al(Department of Endocrinology and Metabolism, The Second People Hospital of Yunnan Province ,Kuraning 650021 ,China)

机构地区：[1]云南省第二人民医院内分泌代谢科,昆明650021 [2]中国医学科学院北京协和医学院,医学生物学研究所

出　　处：《中国糖尿病杂志》2017年第4期289-293,共5页Chinese Journal of Diabetes

基　　金：国家自然科学基金(31660313);云南省生育调节与少数民族优生研究重点实验室项目(ZDSYS2014003)

摘　　要：目的探讨微小RNA(miRNA)多态性位点与中国人群MS的相关性。方法选取581例MS患者和599名健康者。采用TaqMan方法对5个miRNAs的多态性位点(miR-27a rs895819、miR-124a rs531564、miR-128a rs11888095、miR-194a rs3820455、miR-146a rs2910164)分型。评估以上5个多态性位点与MS的相关性。Logistic回归分析miR-124a rs531564和miR-146a rs2910164与MS风险的关系。结果 miR-124a rs531564C可能为MS的保护性等位基因(P=0.01,OR=0.75,95%CI.0.60~0.94)。miR-146a rs2910164C等位基因可能增加患MS的风险(P<0.01,OR=1.55,95%CI:1.31~1.84)。经FPG和胰岛素抵抗指数(HOMA-IR)分别校正后,miR-146a rs2910164 C等位基因仍为MS的危险因素(P<0.01,OR=1.87,95%CI:1.39~2.50;P=0.03,OR=1.55,95%CI:1.05~2.31)。结论miR-124a和miR-146a多态性可能与中国人群MS相关。Objective To investigate the association between single nucleotide polymorphisms （SNPs） located in miRNAs and metabolic syndrome （MS） in Chinese Han population. Methods A total of 581 patients with MS and 599 controls were enrolled in this study. Five SNPs located in miRNAs （rs895819 in miR-27a, rs531564 in miR-124a, rs11888095 in miR-128a, rs3820455 in miR-194a and rs2910164 in miR-146a） were genotyped by TaqMan method. The association between these five SNPs and MS were analyzed and logistic regression model was used for correlation analysis between miR-124a rs531564,miR-146a rs2910164 and MN Results It showed that the C allele of rs531564 in miR-124a may protect against MS （P = 0. 01, OR =0. 75, 95% CI：0. 60 -0. 94）. Conversely, the C allele of rs2910164 in miR-146a may increase the risk of MS （P〈0. 01,OR= 1.55, 95＆CI. 1.31-1.84）. After adjustment for FPG and HOMA-IR respectively,miR-146a rs2910164 C was still a risk allele for MS（P〈0. 01,OR=1.87,95%CI：1. 39-2.50;P=0. 03,0R=1. 55,95%CI：1.05-2.31）. Conclesion The genetic variations in miR-124a and miR-146a are ＇associated with MS in Chinese Han population.

关 键 词：代谢综合征 微小RNA 单核苷酸多态性 

分 类 号：R589[医药卫生—内分泌]