我国5个地区27株人源非O157产志贺毒素大肠埃希菌分子特征初步分析  

Molecular characteristics of human-derived non-O157 Shiga toxin-producing Escherichia coli strains isolated in five regions of China

作　　者：傅珊珊[1] 白向宁[1] 范如岳 许彦梅[1] 许学斌[2] 熊衍文[1] Fu Shanshan Bai Xiangning Fan Ruyue Xu Yanmei Xu Xuebin Xiong Yanwen(State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China Shanghai Municipal Center For Disease Control ＆ Prevention, Shanghai 200336, China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206 [2]上海市疾病预防控制中心,200336

出　　处：《中华微生物学和免疫学杂志》2017年第3期213-218,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（81371762）

摘　　要：目的了解我国5个地区人源性非O157产志贺毒素大肠埃希菌分离株的分子生物学特征。方法对来源于5个地区的27株腹泻患者和健康人的非O157产志贺毒素大肠埃希菌进行血清分型、stx1和stx2基因检测及亚型分析、eae等黏附基因和其他毒力基因检测，并对菌株进行多位点序列分型（MLST）分析。结果27株非O157产志贺毒素大肠埃希菌分为16种O∶H血清型；11株携带stx1a亚型，12株携带stx1c亚型，2株携带stx2e亚型，携带stx1a＋stx2b、stx2d、stx2g亚型各1株；eae、efa1、saa、paa、toxB、astA及ehxA基因的检测率分别为18.5%、18.5%、29.6%、22.2%、11.1%、11.1%及25.9%。MLST将27株菌分为16种不同的ST型。结论我国5个地区人源性非O157产志贺毒素大肠埃希菌菌株在血清型、志贺毒素亚型及毒力基因等方面存在多样性。Objective To understand the molecular characteristics of human-derived non-O157 Shiga toxin-producing Escherichia coil （STEC） strains circulating in five regions of China. Methods Twen- ty-seven non-O157 STEC strains isolated in five geographic regions were investigated by serotyping, StXl/StX2 subtyping and PCR screening for adhesion and other virulence genes. A multilocus sequence typing （MLST） scheme provided by E. coil MLST database were performed to amplify and sequence seven housekeeping genes （adk, icd,fumC, rgyrB, purA, mdh and recA） in those strains. Results Twenty-seven non-O157 STEC strains were typed into 16 O： H serotypes. Among those strains, 11 harbored stx1a, 12 harbored stxlc, two harbored stx2e and the other three strains respectively harbored stx1a＋stx2b, stx2a and stx2s. Positive rates of eae, efal, saa, paa, toxB, astA and ehxA genes were 18.5%, 18.5%, 29.6%, 22.2%, 11. 1%, 11.1% and 25.9% , respectively. The 27 strains were typed into 16 different sequence types （STs） based upon MLST. Conclusion Human-derived non-O157 STEC strains circulating in five regions of China are heterogeneous in their serotypes, stx1/stx2 subtypes and virulence gene profiles.

关 键 词：非O157产志贺毒素大肠埃希菌 志贺毒素 血清分型 多位点序列分型 

分 类 号：R378.21[医药卫生—病原生物学]