对苯二胺-铁(Ⅲ)体系荧光分光光度法检测水样中痕量硫化氢  

Determination of trace hydrogen sulfide in water sample by fluorescence spectrophotometric method with p-phenylenediamine-iron (Ⅲ) system

作　　者：张珊[1] 赵志西[1] 孟亚楠[1] 侯俊峰 李新霞[3] 关明[1] 

机构地区：[1]新疆师范大学化学化工学院,乌鲁木齐830054 [2]新疆阿克苏第十二中学,阿克苏843000 [3]新疆医科大学药学院,乌鲁木齐830000

出　　处：《分析试验室》2017年第4期415-417,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(81260486);新疆研究生科技创新项目基金(XJGRI2015107)资助

摘　　要：建立了对苯二胺-Fe(Ⅲ)体系荧光分光光度法快速有效测定水样中痕量H_2S的新方法。在酸性体系中,对苯二胺与FeCl_3及H_2S反应产生有荧光的劳氏紫,用荧光分光光度法进行测定。其激发波长和发射波长分别为590 nm和620 nm,1.3~65μmol/L,相关系数r=0.9982,检出限为0.122μmol/L,回收率为97.7%~113.2%。方法适用于水样中痕量H_2S含量的测定。Objective： To establish a new method of phenylenediamine-Fe （Ⅲ） system by fluorospectrophotometry quickly determining of trace H2 S valid in water samples. Methods： In acidic systems, p-phenylenediamine reacted with ferric chloride and H2 S produce lloyd＇s purple that is a fluorescent substance with λex = 590 nm and λem = 620 nm. Measured by standard curve and making stability, precision and recovery experimentes were investigated. The linear range is from 1.3 μmol/L to 65 μmol/L, and the correlation coefficient is r = 0. 9982, the detection limit is O.122 μmol/L and the recovery rate is from 97.7% to 113.2%. Fluorescence spectroscopy has the advantages of high sensitivity, and p-phenylenediamine-Fe （ Ⅲ ） system stability, so that the combination of both methods is good stability, simple, sensitive and suitable for rapid determination of trace H2 S content in water sumples.

关 键 词：荧光分光光度法 硫化氢 对苯二胺 劳氏紫 

分 类 号：X832[环境科学与工程—环境工程]