糖皮质激素对变应性鼻炎豚鼠鼻黏膜嗜酸性粒细胞及肥大细胞浸润及P物质含量的影响  

Effect of glucocorticoid on the infiltration of eosinophils,mast cells and SP in nasal mucosa of guinea pigs with allergic rhinitis

作　　者：陈祖尧[1] 罗志葵 朱发梅[1] 郑家法[1] CHEN Zuyao LUO Zhikui ZHU Famei et al(Department of Otorhinolaryngology, Nanhua Hospital Afj：liated to the University of South China, Hengyang 421001, Hunan , China)

机构地区：[1]南华大学附属南华医院耳鼻咽喉头颈外科,湖南衡阳421001 [2]娄底市中心医院耳鼻咽喉头颈外科

出　　处：《中南医学科学杂志》2017年第2期122-126,共5页Medical Science Journal of Central South China

基　　金：湖南省教育厅科研项目(13C803)

摘　　要：目的探索糖皮质激素对变应性鼻炎(AR)豚鼠鼻黏膜嗜酸性粒细胞浸润、肥大细胞浸润及P物质(SP)含量的影响。方法 30只雄性豚鼠分为正常组(对照组)、变应性鼻炎组(模型组)和糖皮质激素治疗组(干预组)(n=10)。采用卵清蛋白腹腔注射基础致敏及双侧鼻腔局部激发建造豚鼠变应性鼻炎模型,干预组用糖皮质激素布地奈德喷鼻治疗。对照组及模型组同期用生理盐水喷鼻处理。豚鼠鼻中隔黏膜予以苏木素—伊红染色、甲苯胺蓝染色及SP免疫组化分析,显微镜下观察计数鼻中隔黏膜中嗜酸粒细胞和肥大细胞浸润程度。光密度半定量分析SP免疫组化染色。结果与对照组比较,模型组症状学评分满足变应性鼻炎诊断标准,有明显嗜酸性粒细胞及肥大细胞浸润;与模型组比较,糖皮质差异激素喷鼻治疗干预后,干预组各项指标和症状均有改善。结论局部应用布地奈德有缓解AR症状,并能降低嗜酸性粒细胞及肥大细胞的浸润程度,减轻组织炎性反应,可能是与通过抑制变应性鼻炎鼻黏膜中SP释放有关。Objective To explore the effect of glucocorticoid on the infiltration of eosinophils, mast cells and substance P （SP） in nasal mucosa of guinea pigs with allergic rhinitis （AR）. Methods 30 of male guinea pigs were divided into nor- mal control group （ NG）, allergic rhinitis groups （MG） and glucocorticoid treatment intervention group （GG） （ n = 10）. Guinea pig model group set up by intraperitoneal injection of ovalbumin sensitization and bilateral nasal local excitation. GG was treated with glucocorticoid budesonide nasal spray treatment for 3 weeks, NG and MG treated with physiological saline na- sal spray processing in the same period.Separation of guinea pigs nasal septum mucosa formaldehyde fixed immediately stained with HE and toluidine blue staining.SP was analysized with immunohistochemical method, and the acidophilic granulocyte and mast cells in nasal septum mucosa were counted under microscope.Immunohistochemical study of substance P average optical density was statistical analyzed. Results compared with the NG, semeiology scores of MG was meet the criteria for the diagnosis of allergic rhinitis,and have obvious eosinophils and mast cells infihration.Compared to the MG, every index and symptom in GG was improved. Conclusion Local application of budesonide can relieve symptoms of AR,reduce tissue inflam- matory reaction,which may relate to inhibit the release of the SP of rhinitis nasal mucosa.

关 键 词：变应性鼻炎 嗜酸性粒细胞 肥大细胞 P物质 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]