三种检测方法在结核感染诊断中的比较研究  

Comparative study of three detection methods in diagnosis of tuberculosis infection

作　　者：张志勇[1] 毛志方[1] 王芹[1] 

机构地区：[1]江西省人民医院,江西南昌330006

出　　处：《基层医学论坛》2017年第7期845-846,共2页The Medical Forum

基　　金：江西省卫生计生委科技计划项目(20155078)

摘　　要：目的比较γ-干扰素释放分析法(IGRA-ELISA)、胶体金法(TB-DOT)以及PCR检测(TB-PCR)在结核感染患者中的诊断效能。方法选择住院高危结核感染病例141例,分别采用IGRA-ELISA、TB-DOT和TB-PCR进行检测,评价三种检测方法的诊断效能。结果 IGRA-ELISA灵敏度89.89%,特异度94.23%;TB-DOT灵敏度86.52%,特异度92.31%;TB-PCR灵敏度73.03%,特异度98.08%。结论IGRA-ELISA技术对结核病诊断方面具有较高的灵敏度和特异度,应用价值显著。Objective To compare the analysis method of IGRA-ELISA release of interferon gamma, colloidal gold （TB-DOT） and PCR （TB-PCR） detection in diagnosis of tuberculosis in the performance of patients. Methods The IGRA-ELISA,TB-DOT and TB-PCR were used in 141 hospitalized patients with high risk of infection combined with to evaluate the diagnostic performance of the three methods. Results The sensitivity of IGRA was 89.89%, and the specificity was 94.23% ;the sensitivity of TB-DOT was 86.52% ,and the specificity was 92.31% ;the sensitivity of TB-PCR was 73.03%, and the specificity was 98.08%.Conclusion IGRA-ELISA technology has high sensitivity and specificity for diagnosis of tuberculosis, obvious application value.

关 键 词：结核感染 诊断 γ-干扰素释放分析 胶体金法 PCR检测 

分 类 号：R52[医药卫生—内科学]