超声辅助离子液体提取反相高效液相色谱法同时测定温郁金中姜黄素和吉马酮的含量  

Simultaneous Determination of Curcumin and Germacrone in Curcuma wenyujin by Ultrasound-Assisted Ionic Liquid-Reversed Phase Liquid Chromatography

作　　者：马铭研[1] 陈维[1] 

机构地区：[1]浙江医药高等专科学校药学院,浙江宁波315000

出　　处：《中国药学杂志》2017年第7期592-596,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金青年科学基金资助项目(21502172)

摘　　要：目的建立以离子液体为提取剂,结合超声辅助提取,利用高效液相色谱法测定温郁金中姜黄素和吉马酮含量的方法。方法采用Agilent ZORBAX Eclipse Plus C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸为流动相,梯度洗脱,流速为1.0 mL·min^(-1),紫外检测波长为265 nm,柱温为30℃。结果提取温郁金中姜黄素和吉马酮的最佳提取条件为0.6 mol·L^(-1),的[BMIM]PF_6甲醇溶液,固液比为1∶20,姜黄素进样量在0.041 2~2.06μg呈良好的线性关系(r=0.999 9),平均回收率为98.31%;吉马酮的进样量在0.010 04~0.502μg内呈良好的线性关系(r=0.999 8),平均回收率为97.62%。结论该方法操作快速简便,绿色环保,重复性好,对温郁金有效成分提取方法创新和质量控制研究具有重要意义。OBJECTIVE To establish a method for the simultaneous determination of curcumin and germacrone in Curcuma wenyu- fin by uhrasound-assisted ionic liquid-reversed phase liquid chromatography. METHODS An Agilent ZORBAX Eclipse Plus Cls column （4. 6 mm × 250 ram, 5 μm） was used. Gradient elution was conducted with the mobile phase consisting of acetonitrile and 0. 1% phos- phoric acid at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 265 nm, the flow rate was set at 1.0 mL · min - 1, and the column temperature was maintained at 30 ℃. RESULTS The result showed that curcumin and germacrone had the highest ex- traction yield when the [ BMIM ] PF6 methanol solution concentration of 0. 6 tool · L-1 was used as the extraction solvent and the solid-liq- uid ratio was 1 ： 20. Under the optimized condition, good linearity of curcumin was shown for curcumin in the range of 0. 041 2 -2. 06 μg （r = 0. 999 9 ）and the average recovery was 98. 31%. The good linearity of germacrone was shown for germacrone in the range of 0. 010 04 -0. 502 μg （r = 0. 999 8）and the average recovery was 97.62%. CONCLUSION This method is convenient, environmentally friend- lygreen and highly reproducible. It is beneficial important to study the extraction method of the active gradients of the traditional Chinese medicine Curcuma wenyujin active gradient and its quality control of Curcuma wenyujin.

关 键 词：温郁金 姜黄素 吉马酮 离子液体 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]