解淀粉芽孢杆菌B9601-Y2对玉米叶斑病的防治效果  

Control Effects of Bacillus amyloliquefaciens 9601-Y2 on Corn Leaf Spots

作　　者：谢兰芬[1,2] 何鹏飞[2] 吴毅歆[2] 肖春[2] 易加红 何月秋[2] 

机构地区：[1]河南科技学院资源与环境学院,河南新乡453003 [2]云南农业大学,农业生物多样性应用技术国家工程中心,昆明650201 [3]昆明市盘龙区阿子营镇农业技术推广站,昆明651711

出　　处：《玉米科学》2017年第2期130-135,共6页Journal of Maize Sciences

基　　金：科技部国际合作项目(2009DFA32360);云南省玉米产业体系项目(20151105560196)

摘　　要：采用凹玻片法测定解淀粉芽孢杆菌B9601-Y2(Y2)发酵液、上清液和芽孢对4种病害的孢子萌发抑制率,研究Y2对玉米叶斑病的防治效果。结果表明,Y2发酵液、上清液和芽孢对大斑病菌抑制率分别为82.72%、76.71%、4.89%,对小斑病菌抑制率分别为88.04%、82.85%、5.63%,对圆斑病菌抑制率分别为84.90%、79.90%、10.81%,对弯孢霉叶斑病菌抑制率分别为98.79%、95.05%和6.23%。温室盆栽试验表明,Y2的最佳施用浓度为1×10~7CFU/m L,最佳施药时间为接种病菌前8 h,对4种叶斑病的预防效果分别达80.83%、84.62%、84.33%和87.74%。田间试验表明,Y2悬浮剂对4种病害的预防效果分别为65.90%、69.21%、67.97%和70.22%。In order to clarify its controlling effect on corn leaf spots, using concave slide method, conidia germi-nation of the fungi（Exserohilum turcicum, Cochliobolus heterostrophus, Cochliobolus carbonumand Curvularia lunata）was determined with fermentation liquid, supernatant and Bacillus spores. The germination inhibition rates were82.72%, 76.71% and 4.89% for E. turcicum; 88.04%, 82.85% and 5.63% for C. heterostrophus; 84.9%, 79.9% and10.81% for C. carbonum; 98.79%, 95.05% and 6.23% for C. lunata, respectively. Through the greenhouse pot exper-iment, the suitable concentration of Y2 was 1×10^7CFU/m L, and the best application time was 8 hours before thepathogen inoculation. Preventive effects for four leaf spot diseases with Y2 fermentation liquid were 80.83%,84.62%, 84.33% and 87.74%, respectively, in the greenhouse. Correspondingly, the biocontrol agent Y2 SC couldprevent 65.90%, 69.21%, 67.97% and 70.22% of the four diseases in the field through its curing effect was not aswell as preventive one.

关 键 词：玉米 玉米叶斑病 大斑病 小斑病 圆斑病 弯孢霉叶斑病 生防菌 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]