河北省冬小麦赤霉病拮抗细菌的分离与鉴定  

Isolation and Identification of Antagonistic Bacteria of Fusarium Head Blight of Winter Wheat in Hebei Province

作　　者：张雪娇[1] 石晶晶[1] 常娜[1] 张雅琴[1] 齐永志[1] 甄文超[1] 尹宝重[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071001

出　　处：《作物杂志》2017年第2期157-162,共6页Crops

基　　金："十二五"国家科技支撑计划项目"粮食丰产科技工程"(2011BAD16B08;2012BAD04B06;2013BAD-07B05);河北省现代农业产业技术体系小麦产业创新团队建设项目(HBCT2013010208)

摘　　要：于2015-2016年小麦季,在河北省15个县(市、区)麦田采集土壤样本115份,采用稀释分离法对土壤样本进行细菌分离,共获得细菌218株。采用平板对峙法进行筛选,获得27株对禾谷镰刀菌(Fusarium graminearum)具有拮抗作用的菌株,平均抑菌率为44.09%;其中XJ-16和LY-2菌株拮抗效果较好,抑菌率分别为73.76%和73.73%。对菌株XJ-16和LY-2进行形态学、生理生化和分子鉴定,确定两菌株均为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。115 soil samples were collected from 15 counties in Hebei province during the wheat growing season from 2015 to 2016. The soil samples were separated by dilution method, then 218 strains of bacteria were obtained. 27 strains with the average inhibition rate of 44.09% on Fusarium were obtained after the filteration of the 218 strains by plate confrontation method. Among them, XJ-16 and LY-2 had good antagonistic effect, the inhibition rates were 73.76% and 73.73%, respectively. The morphological, physiological, biochemical and molecular identification of strain XJ-16 and LY-2 were carried out. The results showed that both two strains were Bacillus amyloliquefaciens.

关 键 词：河北省 小麦 赤霉病 拮抗细菌 分离 鉴定 

分 类 号：S435.121.45[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]