原花青素对多囊卵巢综合征合并动脉粥样硬化模型大鼠的作用  

Effect of Procyanidine on Rat Models of Polycystic Ovary Syndrome Complicated with Atherosclerosis

作　　者：金铉顺[1] 李梦京[1] 金石[1] 张国杰[1] 元奎昌[1] 

机构地区：[1]延边大学附属医院心内科,吉林省延吉市133000

出　　处：《中国全科医学》2017年第12期1463-1468,共6页Chinese General Practice

基　　金：国家自然科学基金资助项目(81460229);吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2012]第18号);延边大学科技发展计划项目[延大科合字(2011)第13号]

摘　　要：目的探究原花青素对多囊卵巢综合征(PCOS)合并动脉粥样硬化模型大鼠的作用及其机制。方法2015年1—10月,采用随机数字表法从20只健康雌性清洁级SD大鼠中选取15只为建模组,在其颈背部皮下埋置双氢睾酮(DHT)缓释片,并且定期更换DHT缓释片的同时,给予高脂饲料配方,给予充分的颗粒饲料和水,建立PCOS合并动脉粥样硬化模型大鼠;其余5只大鼠为对照组,常规饲养。16周后,分别检测两组大鼠体质量、雄激素、瘦素、胰岛素水平,光学显微镜下观察其主动脉内膜病理情况,以验证PCOS合并动脉粥样硬化模型大鼠成功与否。将建模成功的15只大鼠采用随机数字表法分为模型组、辛伐他汀组、原花青素组,各5只。对照组与模型组大鼠给予0.9%氯化钠溶液,辛伐他汀组大鼠给予辛伐他汀片,原花青素组大鼠给予原花青素。治疗4周后,检测4组大鼠脂代谢指标[总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)]水平、脂代谢关键基因[过氧化物酶体增殖物激活受体γ(PPAR-γ)、3-羟基-3-甲基戊二酰辅酶A还原酶(HMG-Co A还原酶)、ATP结合盒转运子A1(ABCA1)、肝脏X受体α(LXR-α)、法尼醇受体(FXR)]mRNA表达水平。结果建模组体质量、雄激素、瘦素、胰岛素水平均低于对照组(P<0.05)。光学显微镜下观察大鼠主动脉内膜病理情况,结果显示,与对照组相比,建模组大鼠主动脉内膜出现大量粥样斑块及胆固醇结晶。模型组TC、TG、LDL水平高于对照组(P<0.05);辛伐他汀组TC、TG、LDL水平低于模型组,HDL水平高于对照组、模型组(P<0.05);原花青素组TC、TG、LDL水平低于模型组,TC、LDL水平低于辛伐他汀组,HDL水平高于对照组、模型组(P<0.05)。模型组PPAR-γ、HMG-Co A还原酶、FXR mRNA表达水平低于对照组(P<0.05);辛伐他汀组PPAR-γ、HMG-Co A还原酶、ABCA1、LXR-α、FXR mRNA表达水平高于模型组,HMG-Co A还原酶、ABCA1�Objective To explore the effect and mechanism of procyanidine on rat models of polycystic ovary syndrome （PCOS）complicated with atherosclerosis. Methods This study was conducted between January and October 2015. For modeling the rats of PCOS complicated with atherosclerosis,we randomly assigned 20 female healthy and clean SD rats to control group （n= 5,conventional feeding）and modeling group 〔n = 15,embedding and regular replacement of dihydrotestosterone （DHT） sustained - release tablets in the subcutaneous tissue of the neck and back and giving enough high - fat food,pellet and water〕. Body weight,androgen,leptin and insulin levels were measured in both groups after 16 weeks of intervention. Aortic tunica intima specimen was taken from both groups and detected by optical microscopy,and the results suggested that the rat models of PCOS complicated with arteriosclerosis were successfully built only in modeling group. Then we randomly divided modeling group into model subgroup,simvastatin subgroup and procyanidine subgroup with 5 rats in each. Both control group and model subgroup were fed with 10 ml/ kg 0. 9% sodium chloride solution,simvastatin subgroup and procyanidine subgroup were fed with 4 mg/ kg simvastatin tablets,50 mg/ kg procyanidine,respectively,once a day,for 4 weeks. At the end of this round of intervention,the rat tail vein blood was taken for measuring the levels of lipid metabolism indicators 〔total cholesterol （TC）,triacylglycerol （TG）,low - density lipoprotein （LDL）and high - density lipoprotein （HDL）〕. Then,all the rats were sacrificed,the carotid artery blood was collected to detect the mRNA expressions of lipid metabolism - associated key genes 〔peroxisome proliferator - activated receptor gamma （PPAR-γ）,3 - hydroxy - 3 - methyl - glutaryl - CoA （HMG-CoA）reductase,ATP -binding cassette transporter A1 （ABCA1）,liver X receptor alpha （LXR-α）and farnesoid X receptor （FXR）〕. Results Modeling group had lower body weight,androgen,lepti

关 键 词：多囊卵巢综合征 动脉粥样硬化 原花青素类 大鼠 辛伐他汀 

分 类 号：R711.75[医药卫生—妇产科学] R543.5[医药卫生—临床医学]