钙结合蛋白Calgranulin A和Calgranulin B在非小细胞肺癌中的表达及临床意义  

Expression and clinical significance of calcium-binding protein Calgranulin A and Calgranulin B in non-small cell lung cancer

作　　者：马威[1] 马超楠[1] 周楠楠[1] 张祎捷[1] 

机构地区：[1]河南大学淮河医院呼吸内科,河南开封100142

出　　处：《中国临床药理学杂志》2017年第7期616-619,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河南省科技厅医学科技攻关基金资助项目(112102310137)

摘　　要：目的研究钙结合蛋白S100A8(Calgranulin A)和S100A9(Calgranulin B)在肺鳞癌及肺腺癌患者中的表达特点及其与临床资料的相关性。方法收集2013年1月至2014年12月进行肺癌根治术的肺鳞癌及肺腺癌患者的肿瘤组织及癌旁正常组织20例、2007年1月至2009年12月确诊为肺鳞癌及肺腺癌患者肿瘤组织石蜡标本100例,癌旁正常组织石蜡标本20例。用聚合酶链式反应(PCR)、蛋白印迹法、免疫组化技术检测S100A8和S100A9在肿瘤组织及癌旁正常组织中的表达水平,并分析其与临床资料的相关性。结果在非小细胞肺癌患者癌组织中,S100A8、S100A9 mRNA阳性表达率为100.0%,与癌旁正常组织中阳性表达率分别为40.0%和85.0%比较显著增高,差异有统计学意义(P<0.05);在非小细胞肺癌患者癌组织中,S100A8、S100A9蛋白阳性表达率均为100.0%,癌旁组织中阳性表达率分别为20.0%,90.0%,癌组织中S100A8蛋白阳性表达水平显著高于癌旁组织,差异有统计学意义(P<0.05)。S100A8在高分化肺腺癌中的表达水平为28.6%,显著低于中低分化肺腺癌的89.5%,差异有统计学意义(P<0.01);Ⅰ期S100A9阳性表达率为73.0%,显著低于Ⅲ/Ⅳ期的100.0%,差异有统计学意义(P<0.001)。结论 S100A8及S100A9在肺腺癌及肺鳞癌患者肿瘤组织中的表达水平高于癌旁正常组织,但是与肺腺癌的分化程度呈负相关,但与肺鳞癌的分化程度无关;S100A9的表达水平与肿瘤TNM分期呈负相关。Objective To evaluate the expression characteristics of calcium-binding protein S100A8（ Calgranulin A） and S100A9（ Calgranulin B） in lung squamous cell carcinoma and lung adenocarcinoma and their correlations with clinical data. Methods Twenty paired snap frozen cancer tissue and para-cancer tissue of lung cancer patients were collected form Jan 2013 to Dec 2014 in our hospital. And other 100 cancer paraffin wax specimen,20 para-cancer paraffin wax specimen were retrospectively collected from Jan 2007 to Dec 2009. The expressions of S100A8 and S100A9 were analyzed by PCR,Western blot and immunohistochemistry assay. The association between S100A8,S100A9 expression and patients clinical characteristics were analyzed.Results Positive rates of S100A8,S100A9 mRNA were 100. 0% for cancer tissue,significant higher than those of para-cancer tissue of40. 0% and 85. 0%（ P 0. 05）. Positive rates of S100A8,S100A9 protein were 100. 0% for cancer tissue,significant higher than those of para-cancer tissue of 20. 0% and 90. 0%（ P 0. 05）. S100A8 positive expression rate was28. 6% and 89. 5% for well differentiation and poor differentiation groups with statistical difference（ P 0. 05）. The expression rate of S100A9 for stage Ⅰ positive was 73. 0% and the 100. 0% of S100A9 for stage Ⅲ/Ⅳ groups with statistical difference（ P 0. 05）. Conclusion The expression levels of S100A8 and S100A9 in lung adenocarcinoma and lung squamous cell carcinoma was higher than those in normal tissue,which were negatively correlated with the degree of differentiation of lung adenocarcinoma,and without correlation to the degree of differentiation of lung squamous cell carcinoma. The expression level of S100A9 was negatively correlated with tumor TNM stage.

关 键 词：Calgranulin A蛋白 Calgranulin B蛋白 肺鳞状细胞癌 肺腺癌 

分 类 号：R979.1[医药卫生—药品]