石菖蒲挥发油有效成分联合左旋多巴对6-羟基多巴诱导帕金森病模型大鼠自噬相关因子影响  

Effect of the Effective Components of Volatile Oil of Acorus Tatarinowii Schott and Levodopa Coadministration on Autophagy Related Factor in 6-hydroxydopamine Induced Parkinson's Rats

作　　者：黄丽平[1,2] 邓敏贞[3] 冯真英 林文新[5] 

机构地区：[1]海南医学院,海南海口571199 [2]岭南师范学院,广东湛江524048 [3]广东省中医院,广东广州510120 [4]湛江市第二人民医院,广东湛江524003 [5]广州市皮肤病防治所,广东广州510095

出　　处：《辽宁中医药大学学报》2017年第4期33-36,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：海南省自然基金项目(20168266);海南省科协青年科技人才学术创新计划项目(HAST201635);海南医学院科研培育基金项目(HY2015-01)

摘　　要：目的:研究石菖蒲挥发油有效成分β-细辛醚联合左旋多巴对6-羟基多巴诱导帕金森病模型大鼠自噬相关因子的影响。方法:将SPF级SD大鼠在内侧前脑束部位注入6-OHDA制备PD模型。将成功的PD模型大鼠随机分为模型组、雷帕霉素组(1 mg/kg)、3-甲基腺嘌呤(3-Methyl adenine,3MA)组(500 nmol/只)、美多巴组(75 mg/kg)、左旋多巴组(60 mg/kg)、β-细辛醚组(15 mg/kg)和联合用药组(左旋多巴60 mg/kg+β-细辛醚15 mg/kg),给药30 d,每天2次。给药末次1 h后,麻醉并灌注各组大鼠,取中脑做免疫荧光和流式检测Beclin-1、LC3B和p62的表达。结果:在免疫荧光和流式细胞术的检测方法中,与假手术组比较,模型组大鼠的Beclin-1和LC3B蛋白表达显著增加(P<0.01),而p62蛋白表达显著减少(P<0.01);与模型组比较,美多巴组、左旋多巴组、β-细辛醚组和联合用药组大鼠的Beclin-1和LC3B蛋白表达显著减少(P<0.05),而p62蛋白表达显著增加(P<0.05),其中β-细辛醚组和联合用药组大鼠的Beclin-1和LC3B蛋白表达显著低于美多巴组和左旋多巴组(P<0.05),而β-细辛醚组和联合用药组大鼠的p62蛋白表达显著高于美多巴组和左旋多巴组(P<0.05)。结论:β-细辛醚与左旋多巴联合应用能减少6-羟基多巴诱导PD大鼠模型中脑的自噬因子Beclin-1和LC3B表达的作用,同时增加p62的表达。Objective：To investigate the effect of the effective components of volatile oil of Acorus tatarinowii Schott and levodopa（L-dopa）co-administration on autophagy related factor in 6-hydroxydopamine induced Parkinson＇s rats. Methods：Severe unilateral lesion of the nigrostriatal pathway was obtained by micro-injection of 6 μL 6-hydroxydopamine（4 μg/μL,Sigma,free based dissolved in a solution of 20 mg/m L L-ascorbic acid in 0.9% normal saline）at a rate of 0.2 μL/min into the medial forebrain bundle（MFB）. Rats were divided into eight groups：sham-operated and model groups;rapamycin group（1 mg/kg）;3-Methyladenine（3-MA）group（500 nmol）;madopar group（75 mg/kg）;L-dopa group（60 mg/kg）;β-asarone group（15 mg/kg）;coadministered group,administered β-asarone ＋ L-dopa（15mg/kg＋ 60mg/kg）. Then Beclin-1,LC3 B and p62 expression in mesencephalon were detected. Results：Beclin-1 and LC3 B expression decreased（P〈0.01）and p62 expression increased significantly（P〈0.01）in madopar,L-dopa,β-asarone and coadministered treated groups compared with that of 6-OHDA model group. Among them,Beclin-1 and LC3 B expression in β-asarone and coadministered treated groups were less than that of madopar or L-dopa treated groups（P〈0.05）,in contrast,p62 expression in β-asarone and coadministered treated groups were higher than that of madopar or L-dopa treated groups（P〈0.05）. Conclusion：The coadministration of β-asarone and L-dopa could decrease Beclin-1 and LC3 B expression and increase p62 expression.

关 键 词：Β-细辛醚 左旋多巴 帕金森病 自噬 

分 类 号：R742[医药卫生—神经病学与精神病学]