大孔树脂纯化五味子总木脂素及其体外还原能力研究  

Study on Purification of Total Lignans from Schisandra Chinensis(Turcz.)Baill by Macroporous Resin and Reducing Ability in Vitro

作　　者：陈晓宇[1] 田振坤[1] 高翔[1] 吴伦[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《辽宁中医药大学学报》2017年第4期39-42,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：黑龙江省教育厅科学技术研究项目(12541736;12541755);黑龙江中医药大学新药研究基金项目(2013xy02);黑龙江中医药大学研究生创新科研项目(yjscx2016047)

摘　　要：目的:以五味子为原料,以总木脂素为研究对象,展开了对总木脂素的提取、分离纯化及还原能力的研究。方法:本文通过静态和动态实验对5种大孔树脂进行筛选,以吸附、解析数据为指标,优选大孔树脂纯化工艺,并利用Fe3+法测定总木脂素体外还原能力。结果:确定AB-8型树脂最优,最佳工艺条件为:五味子总提取物上样浓度为1.5 mg/m L,流速为1 BV/h;最终用50%乙醇(低浓度洗脱剂)和70%乙醇(高浓度洗脱剂)进行洗脱;体外还原能力结果显示:五味子总木脂素在50%和70%乙醇组分对Fe3+表现出较高的还原能力。试验结果显示,通过大孔树脂纯化五味子总木脂素及其体外还原能力的研究,五味子总木脂素的含量从11.68%提高到41.48%。结论:本实验的梯度洗脱法可显著提高五味子中总木脂素的质量分数,并且五味子总木脂素在50%和70%乙醇组分表现出良好的体外还原能力,为今后五味子总木脂素研究提供理论依据。Objective：It is Schisandra chinensis as raw materials and the total lignans for the study that we research the total lignans element of extraction,isolation,purification and reducing power method. Methods：Taking Schizandrin as test index that used the UV spectrophotometry method for measuring the total lignans content from Schisandra chinensis（Turcz）;The static purification condition of lignans were determined on the basis of investigating the adsorption and desorption performance of different kind of macroporous resins. Using Fe3＋ method research the total lignans reducing ability in vitro. Results：AB-8 macroporous resin was chose and employed in this study. The optimal adsorption conditions were as follows：in order to obtain lignin with the density of 1.5 mg/m L. Best elution method is 1 BV/h flow rate in turn with 50% ethanol and 70% ethanol elution. In vitro reducing power results showed that Schisandra lignans showed higher reducing ability in 50% ethanol and 70% ethanol. The test results show that the lignans content of Schisandra crude extracts increased from 11.68% to 41.48%. Conclusion：The method of gradient elution is a better way to purify total lignans Schisandra chinensis（Turcz.）Baill. The total lignans from Schisandra chinensis（Turcz.）Baill in 50% and 70% ethanol component showed good in vitro reduction ability,which provided a theoretical basis on total lignans of Schisandra chinensis（Turcz.）Baill for the future study.

关 键 词：五味子 总木脂素 大孔树脂 还原能力 

分 类 号：R284.2[医药卫生—中药学]