肾动脉移植骨髓间充质干细胞治疗大鼠慢性肾病结果分析  

Bone marrow mesenchymal stem cells transplantation via renal artery for the treatment of chronic nephropathy in rats: analysis of experimental results

作　　者：陆发承 王家平[1] 邢艺苑 万珊杉 韩磊[1] 

机构地区：[1]昆明医科大学第二附属医院放射科,650101

出　　处：《介入放射学杂志》2017年第4期351-354,共4页Journal of Interventional Radiology

摘　　要：目的分析和评价经肾动脉途径移植骨髓间充质干细胞(BMSC)对慢性阿霉素肾病模型大鼠的治疗结果。方法采用50只雄性Sprague-Dawley大鼠作为实验动物。其中2只12周龄大鼠用于BMSC分离培养;12只作为空白对照组(N组),36只经尾静脉注入阿霉素制作慢性肾病大鼠模型,并随机分为慢性肾病对照组(C组)、经肾动脉移植BMSC组(A组)和经尾静脉移植BMSC组(V组),每组12只。N组经尾静脉注入等量0.9%氯化钠溶液。结果 A组、V组、C组在各观察时点血尿素氮、血肌酐、24 h尿蛋白、24 h尿微量蛋白水平均明显高于N组(P<0.01)。移植后1、2周A组24 h尿微量蛋白均低于C组(P<0.01),且血肌酐均较C组和V组低(P<0.01);移植后1周A组24 h尿蛋白、24 h尿微量蛋白均明显低于V组(P<0.01),但2周时与V组差异无统计学意义。结论经肾动脉途径移植BMSC可提高细胞归巢效率,并提高对损伤组织的修复作用。Objective To evaluate the therapeutic effect of bone marrow mesenchymal stem cells （BMSC） transplantation via renal artery in treating experimental rats with adriamycin-induced chronic nephro -pathy. Methods A total of 50 male Sprague-Dawley （SD） rats were used as experimental animals. Two rats were used for the isolation and culture of BMSC. Twelve rats were designed as blank control group （group N）; in other 36 rats adriamycin was injected through caudal vein to establish rat models of chronic nephropathy, these 36 rats were randomly and equally divided into three groups with 12 rats in each group： control group （group C, n=12）, BMSC transplantation via renal artery group （group A, n=12）, and BMSC transplantation via caudal vein group （group V, n=12）. For the rats of group N, the same amount of normal saline was injected through caudal vein. Results At each observation point, the levels of blood urea nitrogen, serum creatinine, 24 h urinary protein and 24 h urinary microprotein in group A,V and C were remarkably higher than those in group N （P〈0.01）. One and two weeks after BMSC transplantation, the 24 h urinary microprotein level in group A was significantly lower than that in group C （P〈0.01）; the serum creatinine level in group A was significantly lower than that in group C and group V （P〈0.01）. One week after BMSC transplantation, both the 24 h urinary protein level and 24 h urinary microprotein level in group A were strikingly lower than those in group V （P〈0.01）, but two weeks after BMSC transplantation these differences between group A and group V became not statistically significant. Conclusion BMSC transplantation via renal artery can improve cell-homing efficiency and improve the repair of damaged tissue as well.

关 键 词：骨髓间充质干细胞 干细胞移植 肾动脉 肾病 

分 类 号：R699.2[医药卫生—泌尿科学]