土大黄游离蒽醌类成分HPLC指纹图谱  

HPLC Fingerprint Analysis of Free Anthraquinones in Rumei Radix et Rhizoma

作　　者：王芳[1,2] 王珍珍[3] 茅向军[2] 许乾丽[2] 林瑞超[4] 戴忠[4] 

机构地区：[1]遵义医学院,贵州遵义563000 [2]贵州省食品药品检验所,贵阳550004 [3]贵州医科大学,贵阳550004 [4]中国食品药品检定研究院,北京100050

出　　处：《中国实验方剂学杂志》2017年第8期72-76,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省中药现代化科技产业研究开发专项(黔科合社字[2009]5003号)

摘　　要：目的:建立不同产地土大黄中游离蒽醌类成分HPLC指纹图谱的共有模式,评价31批不同产地土大黄药材的质量状况。方法:采用高效液相色谱法,Diamonsil C18色谱柱(4.6 mm×200 mm,5μm),以甲醇-0.2%甲酸溶液为流动相进行梯度洗脱,流速1.0 mL·min^(-1),柱温30℃,检测波长254 nm。采用"中药色谱指纹图谱相似度评价系统"(2004 A版)进行相似度评价,采用SPSS 18.0结合聚类分析(cluster analysis,CA)和主成分分析(principal component analysis,PCA)对31批土大黄药材进行质量评价。结果:标定共有指纹峰6个,并指认出大黄素(4号峰),大黄酚(5号峰)和大黄素甲醚(6号峰)3个成分。土大黄指纹图谱的精密度、稳定性、重复性中6个共有峰的相对保留时间与相对峰面积的RSD分别<2.0%,<5.0%。采用聚类分析法和主成分分析法将土大黄药材分为2类,其中有27批药材相似度在0.8以上,进而说明了31批土大黄药材之间存在相似性和差异性。结论:指纹图谱结合化学模式识别的构建有利于全面、准确地控制土大黄药材的内在质量。Objective： To establish a common pattern of HPLC characteristic fingerprint for anthraquinones in Rumei Radix et Rhizoma from different regions, and evaluate the quality of 31 batches of rhubarb from different regions. Method： HPLC was used on diamonsil Cl8 （4.6 mm × 200 mm, 5 μm） column, with methanol and 0.2% formic acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL·min^-1 ;column temperature was 30 ℃ and the detection wavelength was 254 nm. By using Traditional Chinese Medicine Chromatographic Fingorprint Similarity Evaluation System （2004 A ） was used for similarity evaluation, and SPSS 18.0 combined with cluster analysis （CA） and principal component analysis （PCA） was used to evaluate the quality of rhubarb in 31 batches. Result ： Six common fingerprint peaks were found, and three components were identified ： emodin （4^th peak）, chrysophanol （5^th peak） and physcion （6^th peak）. For precision,stability and repeatability of the fingerprint, the average RSD of retention time was less than 2% , and the average RSD of relative peak area was less than 5%. Rumei Radix et Rhizoma was divided into 2 types in CA and PCA methods, and the similarity was above 0. 8 in 27 batches, indicating that there were similarity and difference between 31 batches of rhubarb. Conclusion： Fingerprint combined with chemical pattern recognition is eonducive to a comprehensive and accurate control of the internal quality of Rumei Radix et Rhizoma medicinal materials.

关 键 词：土大黄 特征图谱 聚类分析 主成分分析 

分 类 号：R284.1[医药卫生—中药学]