辣木叶的HPLC指纹图谱  

HPLC Fingerprint of Moringa oleifera Leaves

作　　者：金玲[1] 娄涛涛 刘旋 张旭[1] 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室--省部共建国家重点实验室培育基地,成都611137 [2]西双版纳傣药有限公司,云南景洪666100

出　　处：《中国实验方剂学杂志》2017年第8期86-91,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：成都市科技局惠民项目(2015-HM01-00188-SF)

摘　　要：目的:通过建立辣木叶的HPLC指纹图谱,评价不同产地辣木叶质量的差异,为辣木质量控制和评价提供科学依据,推动辣木的开发与利用。方法:采用HPLC法,Diamonsil Plus C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-0.05%磷酸溶液(B)(0~70 min,5%~30%A;70~75 min,30%~100%A),流速1.0 mL·min^(-1),检测波长230 nm,柱温30℃。测定5个不同产地12批辣木叶的指纹图谱,并采用国家药典委员会颁布的"中药色谱指纹图谱相似度评价系统"(2004 A)和SPSS 21.0统计软件中的聚类分析对指纹图谱进行相似度比较。结果:建立辣木叶指纹图谱共有模式,标定14个指纹图谱共有峰,其中3个成分峰分别为没食子酸、芦丁、槲皮苷,为其有效成分。12批辣木叶中9批的相似度>0.90,说明不同产地及同一产地不同地区辣木叶指纹图谱既有共性又存在一定差异。结论:建立的方法精密度、重复性和稳定性较好,可为辣木叶的鉴别和质量评价提供参考。Objective： To establish the HPLC fingerprint of Moringa oleifera leaves from different for identification and quality control, and promote the development of the modernization process of M. origins oleifera leaves. Method： HPLC was performed on the column of Diamonsil Plus C18 with acetonitrile （A） -0.05% phosphoric acid solution （B） as the mobile phase （0-70 min, 5%-30% A; 70-75 rain, 30%-100% A） at a flow rate of 1.0 mL·min^-1. The detective wavelength was 230 nm and the column temperature was 30℃. The fingerprints of 12 batches of M. oleifera leaves from 5 different origins were compared for similarity by using Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System （2004 A） and the cluster analysis through SPSS 21.0. Result： The common mode for M. oleifera leaves fingerprints was established, and 14 common fingerprint peaks were identified; among them, three component peaks were gallic acid, rutin and quercetin which were the effective components; the similarity was greater than 0.90 in 9 batches of the 12 batches of M. oleifera leaves, indicating that propolis fingerprints had both similarities and differences either from different main producing areas or different fields within the same producing area. Conclusion： This method is reproducible, precise and steady and it can be used for origin identification and quality control of M. oleifera leaves.

关 键 词：辣木叶 高效液相色谱法 指纹图谱 相似度评价 聚类分析 

分 类 号：R284.1[医药卫生—中药学]