化瘀通络灸法对VD大鼠血管内皮细胞增殖迁移变化的影响  

The Effect of Stasis-eliminating and Meridian-unblocking Moxibustion on Vascular Endothelial Cell Proliferation and Migration in VD Rats

作　　者：张曼[1] 杨骏[1] 王萍[2] 王频[1] ZHANG Man YANG Jun WANG Ping WANG Pin(Anhui University of Traditional Chinese Medicine, Hefei 230038,China Ankui University of Traditional Chinese Medicine First Hospital, Hefei 230038,China)

机构地区：[1]安徽中医药大学,合肥230038 [2]安徽中医药大学第一附属医院,合肥230038

出　　处：《上海针灸杂志》2017年第4期467-472,共6页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81473785)

摘　　要：目的体外研究观察化瘀通络灸法治疗后,大鼠血清的效应物质干预血管内皮细胞增值、迁移变化的过程,探讨化瘀通络灸法治疗血管性痴呆可能的促血管生成机制。方法选取Wistar雄性成年大鼠,采用四血管阻断法(4-VO)制备血管性痴呆的大鼠模型,随机分为艾灸组、西药组、模型组,并设假模型组对照,共4组。艾灸组取百会隔附子饼灸,大椎、神庭穴悬灸治疗,西药组以茴拉西坦灌胃,15 d为1个疗程,均为2个疗程。每个疗程结束后,艾灸组、假模型组、模型组大鼠眼眶取血。制备化瘀通络灸法治疗后的动物血清,干预人脐静脉内皮细胞(HUVEC-12)培养过程,另设平行液组,配制含10%胎牛血清(FBS)的RPMI-1640培养液。四甲基唑盐比色法(MTT法)检测内皮细胞的增殖,细胞划痕标记法检测内皮细胞的迁移。结果 2个疗程治疗结束后,通过MTT法检测血管内皮细胞增殖,与模型血清组、假模型血清组、平行液组比较,艾灸血清组大鼠血清干预人脐静脉内皮细胞(HUVEC-12)增殖明显(P<0.01)。2个疗程治疗结束后,通过细胞划痕法检测血管内皮细胞迁移,24 h后与模型血清组、假模型血清组、平行液组比较,艾灸血清组大鼠血清干预HUVEC-12的迁移率升高明显(P<0.01)。结论化瘀通络灸法在改善血管性痴呆大鼠血管内皮细胞增殖、迁移方面的效果明显,从而获取了化瘀通络灸法体外促"血管生成"的有力证据。Objective To investigate the in vitrointervening effect of ratserum effector substances on vascular endothelial cell proliferation and migrationafter stasis-eliminating and meridian-unblocking moxibustion and explore the possible proangiogenic mechanism of stasis-eliminating and meridian-unblocking moxibustion treatment for vascular dementia.Method Male adult Wistar rats were used.Arat model ofvascular dementia was made by four-vessel occlusion（4-VO）.The rats were randomized to moxibustion,Western medicationand model groups.Asham operation group was established as a control.There was a total of four groups.The moxibustion group received aconite cake moxibustion on point Baihui（GV20）and suspended moxibustion on points Dazhui（GV14）and Shenting（GV24） and the Western medication, an oralgavage of aniracetam tablets.Treatment was given 15 days as one course for a total of two courses.Blood was taken from the Orbital cavity in the moxibustion, sham operation and model groups after each course of treatment.After stasis-eliminating and meridian-unblocking moxibustion, animal serum was prepared tointervene in human umbilical vein endothelial cell （HUVEC-12） culture.Another parallel liquid group was set up. RPMI-1640 culture medium containing 10% fetal bovine serum （FBS） was prepared.Endothelial cell proliferationwas examined bymethyl thiazolyl tetrazolium （MTT） colorimetric assay.Endothelial cell migration was examined by cell scratch labeling.Result After two courses of treatment, the examination of endothelial cell proliferation by methyl thiazolyl tetrazolium （MTT） colorimetric assay showed that rat serum intervened significantly in human umbilical vein endothelial cell （HUVEC-12） proliferation in the moxibustion serum group compared with the model serum, sham operation serum and parallel liquid groups （P〈0.01）.At 24 hrs after two courses of treatment, the examination of endothelial cell migrationby cell scratch labelingshowed that rat serum intervention significantly increased

关 键 词：痴呆 血管性 内皮细胞 细胞增殖 细胞迁移 血管生成 大鼠 药饼灸疗法 艾条灸 针灸血清 

分 类 号：R2-03[医药卫生—中医学]