两例输入性登革病毒合并基孔肯雅病毒感染实验室检测  

Identification of Imported Cases of Dual Infection with Dengue Virus and Chikungunya Virus

作　　者：张瑾[1] 徐翮飞[1] 薛晓宁[2] 朱可[1] 陈晓光[1] 张娟[1] 林元[1] 张齐[1] 

机构地区：[1]山东国际旅行卫生保健中心,山东出入境检验检疫局,青岛266071 [2]青岛机场出入境检验检疫局,山东出入境检验检疫局,青岛266071

出　　处：《病毒学报》2017年第2期200-204,共5页Chinese Journal of Virology

基　　金：山东出入境检验检疫局科研项目(项目号:SK2014030)~~

摘　　要：对不明原因发热病人开展登革病毒和基孔肯雅病毒合并感染的检测,明确合并感染的可能性,为防御这两种虫媒传染病的传播提供依据。通过流行病史调查,临床症状和实验室检测对两名从泰国普吉岛旅行归来的发热病人进行了登革病毒和基孔肯雅病毒感染的诊断。两名患者在泰国旅行停留10天,在此期间两人均有蚊虫叮咬史。回国3天后两患者相继出现高热症状(39℃以上),且伴有皮疹,肌肉酸痛和乏力。实验室血常规检测发现轻度血小板降低伴淋巴细胞减少,登革病毒IgG/IgM快速诊断试剂盒检测发现一名患者登革病毒IgM阳性。实时荧光RT-PCR检测证实两患者血液中登革病毒和基孔肯雅病毒核酸均阳性,同时用RT-PCR方法扩增获得了登革病毒C-prM蛋白部分基因,经测序和同源性分析,证实感染登革病毒属于Ⅰ型登革热病毒。这是我国首次出现的输入性登革病毒合并基孔肯雅病毒感染病例,本研究建议对流行病史和临床症状满足的病例要同时进行两种病毒的实验室检测。We wished to identify dual infection with Dengue virus and Chikungunya virus in patients with fever of unknown. An investigation （epidemiology, symptoms and laboratory tests） of two cases with acute fever was carried out. Diagnostic methods included quantitative real-time reverse-transcription poly- merase chain reaction （qRT-PCR） ; Molecular characterization was based on the sequences of partial envelope genes. Two travelers had been exposed to mosquitoes in Thailand （an epidemic area of dengue virus and Chikungunya virus）. High fever, arthralgia and myalgia had been reported in the few days after their return. A mildly low platelet count and mild leukopenia was noted . Antibody detection with a Dengue IgG/IgM Combo Test Card showed IgM positivity in the blood of one patient, qRT-PCR showed positive reactions to dengue virus and chikungunya virus , which was classified using sequencing to DENV-1. This is the first report of imported co-infection by dengue virus and ehikungunya virus in China. We suggest that co-infection should be surveyed in people showing the clinical signs of a dengue fever-like disease.

关 键 词：登革病毒 基孔肯雅病毒 合并感染 输入性 

分 类 号：R373.3[医药卫生—病原生物学]