RFFIT、FAVN、ELISA及狂犬病中和抗体检测试剂盒对狂犬病抗体检测的比较研究  

Comparative Study on the Detection of Rabies Antibodies using RFFIT,FAVN,ELISA and RVNA kits

作　　者：于鹏程[1] 由淑珍 卢学新[1] 陶晓燕[1] 刘淑清[1] 纪军 朱武洋[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206 [2]北京博雅晟康医学科技有限公司,北京100872

出　　处：《病毒学报》2017年第2期265-268,共4页Chinese Journal of Virology

基　　金：国家重点研发计划项目(项目号:2016YFD0500400),题目:重要神经嗜性人兽共患病免疫与致病机制研究;国家科技重大专项项目(项目号:2014ZX10004002),题目:艾滋病和病毒性肝炎等重大传染病防治;国家科技支撑计划(项目号:2014BAI13B04),题目:狂犬病净化技术集成与人狂犬病消除示范研究;国家自然科学基金(项目号:31500152),题目:我国狂犬病病毒种群划分系统的建立

摘　　要：为了比较RFFIT、FAVN、ELISA及狂犬病中和抗体检测试剂盒(RVNA Kit)等四种方法对狂犬病抗体的检测效果,对65份狂犬病疫苗免疫前、免疫后临床血清样本,分别使用RFFIT、FAVN、ELISA及RVNA Kit方法对狂犬病病毒特异性抗体进行测定,并对检测结果的阳性符合率、相关性及重复性进行分析。检测结果显示,RFFIT、FAVN及RVNA kit的阳性符合率为100%,三种方法与ELISA试剂盒的阳性符合率为95.38%,对0.5IU/mL^10IU/mL的血清样本进行阳性符合率分析显示,四种方法的阳性符合率为100%;相关性研究结果表明,RFFIT、FAVN及RVNA Kit之间任意两种方法检测结果的相关系数均超过0.97,但ELISA与其他三种方法检测结果的相关系数均低于0.86;同一份样品的重复性检验结果显示,RVNA Kit和ELISA比RFFIT和FAVN具有更好的重复性,而RVNA Kit的重复性最好,其变异系数仅6%。研究结果表明,RFFIT、FAVN及RVNA Kit等三种方法可通用于血清中抗狂犬病病毒中和抗体的检测,而ELISA检测结果与中和抗体效价有一定差距,但在经过优化、校验后ELISA也可以在有效范围内用于狂犬病抗体的检测。We wished to compare the detection ability of rabies antibodies using the rapid fluorescent focus inhibition test（RFFIT）,fluorescent antibody virus neutralization test（FAVN）,enzyme linked immunosorbent assay（ELISA）and rabies virus neutralizing antibody（RVNA）kits.The rabies-virus specific antibody of 65 human serum samples before and after immunization with a rabies vaccine was detected using these four methods.Then,the positive coincidence rate,correlation and stability of the results were analyzed.Comparison of the RFFIT,FAVN,and RVNA kits revealed a positive coincidence rate of100%.Comparison of the above three methods with the ELISA kit revealed a positive coincidence rate of95.38%.For the 0.5-10IU/mL serum samples,there was a positive coincidence rate of 100% between the four methods.The correlation coefficient for the detection results between two methods for RFFIT,FAVN and RVNA kits was〉 0.97.However,the correlation coefficient for the detection results of ELISA compared with those of the other three methods was 0.86.Stabilization of the same sample showed that,compared with RFFIT and FAVN,RVNA and ELISA had better stability,and that the RVNA kit was the best.These results show that RFFIT,FAVN and RVNA kits can be used for the detection of anti-rabies virus-neutralizing antibodies in serum and that,after optimization and calibration,ELISA can be used for the detection of rabies antibodies in the effective range.

关 键 词：RVNA KIT RFFIT ELISA FAVN 

分 类 号：R373.9[医药卫生—病原生物学]