温肾降浊散对大鼠阳离子化牛血清白蛋白肾小球肾炎模型的影响  

The Influence of Spleen-Warming and Turbidity-Removing Powder on C-BSA Glomerulonephritis Model of Rats

作　　者：牛春影 刘国良[1] 李艳薇[1] 胡中花 刘海洋[1] 刘斌[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《河南中医》2017年第4期590-593,共4页Henan Traditional Chinese Medicine

基　　金：黑龙江省自然科学基金项目(编号:H201328);黑龙江省教育厅科学技术研究项目(编号:12541758);中央级公益性科研院所基本科研业务专项(编号:YZYN-13-02);黑龙江中医药大学校级基金(编号:2012-035082)

摘　　要：目的:观察温肾降浊散对大鼠阳离子化牛血清白蛋白(Cationic bovine serum albumin,C-BSA)肾小球肾炎模型的影响。方法:随机选取10只为正常对照组,其余大鼠制备C-BSA肾小球肾炎模型,造模成功的大鼠随机分为模型对照组9只、雷公藤多苷治疗组8只、温肾降浊散高剂量组7只和温肾降浊散低剂量组9只。温肾降浊散高剂量组给予3.3 g·(kg·d)-1混悬液灌胃,低剂量组给予0.33 g·(kg·d)-1混悬液灌胃,每日1次。雷公藤多苷治疗组给予10 g·(kg·d)-1混悬液灌胃,每日1次。正常对照组和模型对照组灌胃给予等量蒸馏水,至实验第63天。取大鼠全血和尿液标本,分别做24 h尿蛋白定量、血尿素氮(blood urea nitrogen,BUN)、肌酐(serum creatinine,Scr)、三酰甘油(three acyl glycerin,TG)、血清总胆固醇(total cholesterol,TC)及低密度脂蛋白胆固醇(low density lipoprotein,LDL-C)的测定。结果:与正常对照组比较,模型对照组24 h尿蛋白、Scr、TC、TG、BUN和LDL均升高,其中24 h尿蛋白、Scr、TC和TG具有显著性差异(P<0.05),BUN、LDL无显著性差异(P>0.05)。与模型对照组比较,雷公藤多苷治疗组、温肾降浊散高剂量组及低剂量组24 h尿蛋白、Scr、TC、TG、BUN、LDL均降低,其中24 h尿蛋白、Scr、TC、TG具有显著性差异(P<0.05),BUN、LDL无显著性差异(P>0.05)。与雷公藤多苷治疗组比较,温肾降浊散高剂量组及低剂量组各指标均无显著性差异(P>0.05),作用与雷公藤多苷相当。结论:温肾降浊散对大鼠C-BSA肾小球肾炎模型有较好的治疗作用。Objective： To observe the clinical curative effect of The Influence of Spleen-Warming and Turbidity-Removing Powder on cationic bovine serum albumin（ C-BSA） glomerulonephritis model of rats. Methods： Ten rats were randomly chosen into the normal control group,and the left ones were prepared to establish（ C-BSA） glomerulonephritis models. The successful model rats were randomly divided into the model group with 9 cases,the ripterygium wilfordii treatment group with 8 cases,Turbidity-Removing Powder high-dosage group with 7cases,and Turbidity-Removing Powder low-dosage group with 9 cases. The ones in TurbidityRemoving Powder high-dosage group were given suspension injection for gavage in the form of 3. 3 g·（ kg·d）-1,while the ones in Turbidity-Removing Powder low-dosage group were given suspension injection for gavage in the form of 0. 33 g·（ kg·d）-1,once daily. The ones in the ripterygium wilfordii treatment group were given suspension injection for gavage in the form of10 g·（ kg·d）-1,once daily. The ones Normal control group and model control group were given distilled water for sixty-third days. Then the whole blood and urine samples of rats were taken to determinate 24-h urine protein,blood urea nitrogen（ BUN）,serum creatinine（ Scr）,three acyl three glycerin（ TG）,serum total cholesterol（ TC） and low density lipoprotein cholesterol（ LDL-C） respectively. Results： Compared with the normal control group,the model control group＇s 24-h urinary protein,Scr,TC,TG,BUN and LDL were all increased; Among of them,the differences in 24-h urine protein,Scr,TC and TG had significant difference（ P〉0. 05）,but BUN and LDL had no significant difference（ P〉0. 05）. Compared with the model group,24-h urinary protein,Scr,TC,TG,BUN,LDL of the ripterygium wilfordii treatment group,Turbidity-Removing Powder high-dosage group,and Turbidity-Removing Powder low-dosage group were all decreased,Among of them,the differences in 24-h urine protein,Scr,TC and TG had significant di

关 键 词：温肾降浊散 阳离子化牛血清白蛋白 肾炎模型 大鼠 

分 类 号：R285.5[医药卫生—中药学]