参松养心胶囊对血管紧张素Ⅱ引起的心肌细胞凋亡的影响  

The Influence of Shensong Yangxin Capsules on Myocardial Cell Apoptosis due to Angiotensin Ⅱ

作　　者：马彦卓[1] 孔令锋[1] 南凯 王冬梅[1] 

机构地区：[1]中国人民解放军白求恩国际和平医院,河北石家庄050000 [2]河北省卫生医学科技发展研究中心,河北石家庄050051

出　　处：《河南中医》2017年第4期602-604,共3页Henan Traditional Chinese Medicine

基　　金：河北省应用基础研究重点基础研究项目(编号:13967710)

摘　　要：目的:观察参松养心胶囊对血管紧张素Ⅱ(AngⅡ)引起的心肌细胞凋亡的影响。方法:分离大鼠乳鼠心肌细胞,随机分为对照组(PBS)、AngⅡ(0.1μmol·L^(-1))组、参松养心胶囊(10μmol·L^(-1))预处理1 h+AngⅡ组;Mn(Ⅲ)TBAP(2.5μmol·L^(-1))预处理1 h+AngⅡ组。通过MTT和Caspase-3/7活性检测试剂盒检测细胞活性和细胞凋亡变化;利用western-blot检测心肌细胞SOD-1和Nox2/gp91phox表达。结果:与对照组比较,AngⅡ引起心肌细胞活性明显下降,凋亡细胞显著增加,心肌细胞SOD-1表达下调,而Nox2/gp91phox表达上调。参松养心胶囊预处理后,再给予AngⅡ干预,与AngⅡ处理组比较,心肌细胞活性明显增加,凋亡细胞显著减少,心肌细胞SOD-1表达上调,而Nox2/gp91phox表达下调。Mn(Ⅲ)TBAP作为超氧自由基的清除剂,与参松养心胶囊胶囊作用类似,同样可以抑制AngⅡ引起的细胞损伤。结论:参松养心胶囊通过抑制心肌细胞氧化应激反应,减轻AngⅡ引起的细胞损伤。Objective： To observe the influence of Shensong Yangxin Capsules（ SSYXC） on myocardial cell apoptosis due to angiotensin Ⅱ. Methods： The chosen myocardial cells of infantile rats were separated,and were randomly divided into the control group（ PBS）,the Ang Ⅱ（ 0. 1 μmol·L^-1） group,SSYXC（ 10 μmol·L^-1） pretreatment ＋ Ang Ⅱgroup,the Mn（Ⅲ） TBAP（ 2. 5μmol·L^-1） pretreatment 1 h ＋ Ang Ⅱ group. MTT and Caspase-3/7 activity detection kit,the changes of cell viability and apoptosis were detected. By using western-blot,the expression of SOD-1 and Nox2/gp91 phox were detected. Results： Compared with the control group,Ang Ⅱ significantly decreased the activity of myocardial cells,the apoptotic cells increased significantly,the expression of SOD-1 was down-regulated,and the expression of Nox2/gp91 phoxwas up-regulated. After pretreatment with SSYXC plus Ang Ⅱ intervention,compared with the Ang II treatment group,the activity of myocardial cells increased,apoptotic cells decreased significantly; the expression of SOD-1 was up-regulated,while that of Nox2/gp91 phoxwas down-regulated. Mn（ Ⅲ）TBAP as superoxide radical scavenger,it has similar function to that of SSYXC,can also inhibit the cell injury caused by Ang Ⅱ.Conclusion： SSYXC can reduce the cell damage caused by Ang II by inhibiting the oxidative stress in myocardial cells.

关 键 词：参松养心胶囊 血管紧张素Ⅱ 心肌细胞凋亡 氧化应激 乳鼠 

分 类 号：R285.5[医药卫生—中药学]