酵母挥发性物质环辛四烯抑制灰霉菌的研究  

Inhibitory Effect of the Yeast Volatile Compound Cyclooctatetraene on Botrytis cinerea

作　　者：黄蓉[1] 胡建坤[1] 黄瑞荣[1] 

机构地区：[1]江西省农业科学院植物保护研究所,南昌330200

出　　处：《中国生物防治学报》2017年第2期266-272,共7页Chinese Journal of Biological Control

基　　金：国家自然科学基金(31201562);公益性行业(农业)科研专项(201303025);江西现代农业科研协同创新专项(JXXTCX2015005-004);江西省现代农业蔬菜产业体系病虫害防治岗位专项(JXARS-06);江西省重点研究计划项目(20161ACF60015)

摘　　要：为了探明生防酵母菌菌株C410产生的挥发性物质对灰霉菌的抑制作用机理,本文以挥发性物质的主要成分之一环辛四烯为研究对象,测定其对灰霉菌的糖、蛋白、DNA、RNA、细胞的形态及细胞膜通透性的影响。结果表明,在16.5μL/L环辛四烯的作用下,灰霉菌生长速率显著降低,总糖含量基本不变;还原糖含量显著降低;蛋白含量变化不显著;RNA的完整性显著降低。在49.5μL/L环辛四烯的作用下,灰霉菌的生长几乎完全停止,但细胞通透性不受影响,不导致细胞死亡。环辛四烯孵育下灰霉菌DNA分子没有被打断。由此推测还原糖和RNA代谢过程是环辛四烯作用于灰霉菌的重要靶标,抑制其生长及侵染。In order to determine the inhibitory effect of volatile organic compounds produced by biocontrol yeast strain C410 onBotrytis cinerea, cyclooctatetraene, one of the dominant components of volatile organic compounds, was used to investigate its effect on sugar, protein, DNA, RNA, cell morphology and cell membrane permeability of B. cinerea. The results showed that when B. cinerea was treated with 16.5 μL/L cyclooctatetraene, the growth rate, the reducing sugar content and the RNA integrity were significantly decreased, while the total sugar content was barely changed and the protein content was declined slightly. When treated with 49.5 μL/L cyclooctatetraene, B. cinerea stopped growing, but the cell permeability was not affected and cells would not die. DNA was not destroyed by cyclooctatetraene. Therefore, the synthesis and metabolism of reducing sugars and RNA may be two important targets for cyclooctatetraene to suppress the pathogen growth and infection.

关 键 词：环辛四烯 灰霉菌 抑制 机理 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]