LC-PDA-MS/MS法分析酒石酸阿福特罗中的有关物质  

Identification of Related Substances in Arformoterol Tartrate by LC-PDA-MS/MS

作　　者：韩睿[1] 杭太俊[2] 胡楠[1] 蒋艳[1] 郑小刚[1] 

机构地区：[1]常州市第一人民医院药事科,江苏常州213003 [2]中国药科大学药学院药物分析教研室,江苏南京210009

出　　处：《中国医药工业杂志》2017年第4期553-558,共6页Chinese Journal of Pharmaceuticals

摘　　要：采用液相色谱-质谱联用技术检测酒石酸阿福特罗粗品中的有关物质。采用Sepax Amethyst-C_8色谱柱,以乙腈∶含0.3%乙酸铵的1%甲酸水溶液为流动相,梯度洗脱;采用LC-二极管阵列检测器(PDA)测定各有关物质的UV吸收,乙腈辅助电喷雾正离子化的LC-MS/MS法测定各有关物质的母离子和子离子,并进行解析,推测出7个主要有关物质的结构,通过与已知化合物对照品进行HPLC比对,确证了Ⅰ、Ⅲ和Ⅳ的结构。An LC-MS/MS method was established for the identification of the related substances in the crude product of arformoterol tartrate. A Sepax Amethyst-C8 column was used, with the mobile phase of acetonitrile∶1%formic acid aqueous solution containing 0.3%ammonium acetate for gradient elution. The UV absorption of each related substance was detected by LC-PDA, while the parent and the product ions were acquired by LC-MS/MS with acetonitrile aided electro-spray positive ionization respectively. Seven related substances were identified by LC-MS/MS and the structures of three compounds （Ⅰ, Ⅲ and Ⅳ） were confirmed by comparasion of the HPLC behaviors with the references.

关 键 词：酒石酸阿福特罗 有关物质 对映异构体 液相色谱-串联质谱 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]