ICP-MS法同时测定人工蛹虫草中4种重金属元素的含量  

Simultaneous Determination of Contents of 4 Heavy Metal Elements in Cultured Cordyceps militaris by ICP-MS

作　　者：张勇[1] 卓宝松 刘宝岩 康文艺[1] 

机构地区：[1]黄河科技学院纳米研究所,郑州450063 [2]濮阳市天元虫草研究有限公司,河南濮阳457000

出　　处：《中国药房》2017年第12期1684-1686,共3页China Pharmacy

基　　金：河南省产学研项目(No.162107000038);河南省高等学校重点科研项目(No.17B360005)

摘　　要：目的:建立同时测定人工蛹虫草中4种重金属元素含量的方法。方法:采用硝酸加热法消解样品,采用电感耦合等离子体质谱法进行测定:射频功率为1.15 k W,采样深度为65 mm,辅助气流速为1.0 L/min,冷却气体流量为13.0 L/min,环境温度为25℃,自动等离子体观测。结果:砷、镉、汞、铅检测质量浓度线性范围分别为0~20μg/m L(r=0.997 0)、0~10μg/m L(r=0.999 5)、0~5μg/m L(r=0.995 5)、0~20μg/m L(r=0.996 0);检测限分别为0.128、0.003、0.002、0.004 mg;精密度、稳定性、重复性试验的RSD<6.5%;加样回收率分别为90.4%~100.6%(RSD=3.45%,n=9)、94.3%~101.3%(RSD=2.93%,n=9)、90.0%~102.3%(RSD=4.03%,n=9)、92.3%~103.0%(RSD=3.53%,n=9)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于人工蛹虫草中4种重金属元素含量的同时测定;所测10批人工蛹虫草中As、Cd、Hg、Pb 4种重金属元素含量均符合国家相关标准。OBJECTIVE： To establish the method for the simultaneous determination of contents of 4 heavy metal elements in cultured Cordyceps militaris. METHODS： The samples were digested by nitric acid heating method and then determined by ICP-MS including radio-frequency power of 1.15 kW, sampling depth of 65 nm, auxiliary gas flow rate of 1.0 L/rain, cooling gas flow of 13.0 L/min, temperature of 25 ℃, automatic plasma. RESULTS： The linear ranges of arsenic （As） ,cadmium （Cd）, mercury （Hg） and lead （Pb） were 0-20 gg/mL（r=0.997 0）, 0-10 gg/mL（r=0.999 5）, 0-5 gg/mL（r=0.995 5）, 0-20 btg/mL（r= 0.996 0）, respectively. Detectio limit were 0.128,0.003,0.002,0.004 mg. RSDs of precision, stability and reproducibility tests were all lower than 6.5%. Recoveries of them were 90.4% -100.6% （RSD=3.45% , n=9） , 94.3% -101.3% （RSD=2.93% , n=9）, 90.0%-102.3% （RSD=4.03%, n=9）, 92.3%-103.0% （RSD=3.53%, n=9）, respectively. CONCLUSIONS ： The method is simple, precise, stable, reproducible, and can be used for simultaneous determination of contents of 4 heavy metal elements in cultured C. militaris. The contents of As, Cd, Hg and Pb in 10 batches of sample are all in line with related national standards.

关 键 词：人工蛹虫草 重金属 电感耦合等离子体质谱 

分 类 号：R927.2[医药卫生—药学]