响应面优化微波-超声波协同提取灵芝三萜工艺及其清除自由基活性  

Response surface optimization of microwave-ultrasound extraction technology and free radical scavenging activities of Ganoderma lingzhi triterpenoids

作　　者：梁磊[1] 黄清铧 王庆福[1] 安玉兴[1] 李奇伟[1] 张柳莲 康佩姿[1] 

机构地区：[1]广东省生物工程研究所(广州甘蔗糖业研究所)广东省生物质高值化利用工程实验室广东省甘蔗改良与生物炼制重点实验室广州市植物纤维综合利用重点实验室,广东广州510316

出　　处：《菌物学报》2017年第4期512-521,共10页Mycosystema

基　　金：国家自然科学基金(51678174);广州市珠江科技新星项目(2014J2200074);广东省科技项目(2013B060600001;2015A0107008);广东省科学院专项资金项目(2016GDASPT-0108;2017GDASCX-0105)~~

摘　　要：探索微波-超声波协同提取仪对灵芝三萜的提取工艺及提取产物的抗氧化活性,在单因素实验的基础上,选择微波功率、超声波功率、乙醇浓度和提取时间4个因素,通过响应面分析进行灵芝三萜提取工艺的优化。结果显示,在乙醇含量75%、微波功率650W、超声波功率730W、提取时间20min时灵芝三萜的提取率达13.18mg/g,其中灵芝酸A含量为2.5mg/g;抗氧化活性结果表明,灵芝三萜粗提物对DPPH清除效果优于茶多酚。The extraction technology and free radical scavenging activities of Ganoderma lingzhi triterpenoids were studied by use of microwave-ultrasound extraction process. Based on single factor experiment, response surface methodology was used to optimize the process parameters. The effects of extraction duration, ultrasonic power, microwave power, and ethanol content were investigated. Results showed that the optimum extraction conditions were extractive duration 20 min, ultrasonic power 730W,microwave power 650W and ethanol content 75%.Under the optimized conditions,the Ganoderma lingzhi triterpenoid extraction rate was 13.18mg/g,and ganoderma acid A was 2.5mg/g.DPPH scavenging effect shows that Ganoderma lingzhi triterpenoid is better than tea polyphenol.

关 键 词：灵芝三萜 提取工艺 微波-超声波协同 响应面 

分 类 号：R284[医药卫生—中药学]