BS^3交联分析β淀粉样蛋白的寡聚结构  

BS^3 crosslinking analysis of the state of β-amyloid aggregate

作　　者：石镜明[1] 贺学[1] 廉会娟[1] 吴涛[1] 武美娜[1] 孙正启[1] 

机构地区：[1]西藏民族大学医学院/高原环境与疾病相关基因研究实验室,陕西咸阳712082

出　　处：《西北大学学报（自然科学版）》2017年第2期222-226,共5页Journal of Northwest University（Natural Science Edition）

基　　金：西藏自治区自然科学基金资助项目(12KJZRZMY02;2015zr-13-12)

摘　　要：使用交联剂双琥珀酰亚胺辛二酸酯磺酸钠盐(Bis(sulfosuccinimidyl),BS3)对Aβ1-42进行交联,通过与之前报道的戊二醛交联进行比较分析,可以看出BS3更适合分析蛋白的寡聚状态,这将为Aβ蛋白及类似的易聚集蛋白在体内、外研究提供很好的参考。An agent named bis（sulfosuccinimidyl） （BS3） is used to cross-link Aβ1-42. Compared with glut- araldehyde which was previously reported as an Aβ cross-linking agent, it is not difficult to find that BS3 is more suitable for the analysis of protein in extra-membrane Aβ oligomers states, which will provide a good ex- perimental method to analyze Aβ protein and similar easy aggregation protein in vivo and vitro studies.

关 键 词：双琥珀酰亚胺辛二酸酯磺酸钠盐（BS^3） Β淀粉样蛋白 寡聚体 

分 类 号：Q518.4[生物学—生物化学]